Is the antibody always to blame?-标准-资讯-生物在线
·

Is the antibody always to blame?

作者：Agrisera AB 2020-10-21T00:00 (访问量:1872)

 Is the antibody always to blame? 

In the example blot to the right, the same antibody was used (Anti-BAK1, AS12 1858) but the proteins were extracted with two different buffers. 
Minor adjustments in the buffer components resulted in the improved detection of the target protein - from no band detected at all as seen in the left panel to a band of correct MW visible in the right panel. 

If you are interested to learn more how to optimize your western blot, you are welcome to contact us or request a copy of the Agrisera Western Blot Guide.
 


 

   Western blot using anti-BAK1 antibodies
 

 

 

 

Agrisera AB 商家主页

地 址： 21 Vännäs, SWEDEN, Europe

联系人： Dr. Joanna Porankiewicz-Asplund

电 话： 0046704137107

传 真：

Email：61382170@qq.com

相关咨询

Antigen, immunogen and epitope, what is the difference? (2022-05-24T10:12 浏览数:4644)

How to transfer proteins of low molecular weight? (2022-05-24T10:11 浏览数:4784)

How to save time and shorten Western blot protocol? (2022-05-24T10:09 浏览数:3144)

How to get an intense signal in Western blot using a weak antibody? (2022-04-14T17:01 浏览数:7163)

Antigen, immunogen and epitope, what is the difference? (2022-04-14T17:00 浏览数:6919)

What are the benefits of using directly conjugated primary antibodies? (2022-04-14T16:59 浏览数:5437)

How to transfer proteins of low molecular weight? (2022-04-14T16:57 浏览数:4746)

20% discount on loading control and compartment marker antibodies (2022-04-14T16:57 浏览数:4785)

New epitope tag antibody mNeonGreen, detects mNG tag expressed in plant and algal vectors (2022-02-23T10:28 浏览数:799)

New epitope tag antibody mNeonGreen, detects mNG tag expressed in plant and algal vectors (2022-02-23T10:28 浏览数:4081)

ADVERTISEMENT