荧光染料 |
Ex(nm) |
Em(nm) |
FITC |
490 |
525 |
TRITC |
547 |
572 |
Cy2 |
489 |
506 |
Cy3 |
555 |
570 |
Cy5 |
646 |
620 |
Cy5.5 |
675 |
694 |
Cy7 |
750 |
773 |
Calcein |
494 |
517 |
Rhodamine 110 |
496 |
520 |
Rhodamine B |
555 |
580 |
5-Carboxy Fluorescein |
492 |
518 |
5-FAM, SE |
492 |
520 |
6-TAMRA SE |
543 |
575 |
6-Carboxy-X-rhodamine |
568 |
590 |
Alexa TM 488 |
490 |
520 |
BODIPY |
530 |
550 |
Alexa TM 546 |
555 |
570 |
Pyronin Y |
550 |
580 |
Thiadicarbocyanine |
651 |
670 |
Alexa TM 594 |
590 |
615 |
6-Aminofluorescein |
490 |
520 |
Dansyl chloride |
337 |
492 |
Rhodamine 123 |
507 |
529 |
4-Methylumbelliferone |
385 |
502 |
5-carboxy-2,7-dichlorofluorescein |
504 |
529 |
5-Carboxynapthofluorescein |
512 |
568 |
5-Carboxytetramethylrhodamine (5-TAMRA) |
542 |
568 |
5-FAM (5-Carboxyfluorescein) |
492 |
518 |
5-ROX (carboxy-X-rhodamine) |
567 |
591 |
5-TAMRA (5-Carboxytetramethylrhodamine) |
548 |
552 |
Acriflavin |
436 |
520 |
Alexa Fluor 350™ |
346 |
442 |
Alexa Fluor 430™ |
431 |
540 |
Alexa Fluor 488™ |
492 |
520 |
Alexa Fluor 532™ |
531 |
553 |
Alexa Fluor 568™ |
577 |
603 |
Alexa Fluor 633™ |
632 |
650 |
Alexa Fluor 680™ |
679 |
702 |
AMCA(Aminomethylcoumarin) |
345 |
425 |
Aminocoumarin |
346 |
442 |
Anthrocyl stearate |
381 |
446 |
APC-Cy7 |
655 |
755 |
Atabrine |
436 |
490 |
Aurophosphine |
490 |
515 |
Bisbenzamide |
360 |
461 |
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