肿瘤坏死因子受体超家族成员4(TNFRSF4)重组蛋白说明书
Recombinant Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4)
[ PROPERTIES ]
Residues: Lys29~Gly210
with N-terminal His-Tag.
Host: E. coli
Subcellular Location: Membrane; Single-pass
type I membrane protein. Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 7.5
Predicted Molecular Mass: 21.7kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in ddH2O
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37ºC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-KH TYPSGHKCCR ECQPGHGMVS RCDHTRDTLC HPCETGFYNEAV NY D TCKQC TQ CNHRSGSE LKQN CTPTQD TV C RCRPG TQ PR QDSGYK LGVDCVPCPPGH FSPGNNQACK PWTNCTLSGK QTRHPASDSL DAVCEDRSLL ATLLWETQRPTFRPTTVQST TVWPRTSELP SPPTLVTPEG
[ REFERENCES ]
1. Calderhead D.M., et al. (1993) J. Immunol. 151:5261-5271.
2. Birkeland M.L., et al. (1995) Eur. J. Immunol. 25:926-930.
3. Pippig S.D., et al. (1999) J. Immunol. 163:6520-6529.
4. Kopf M., et al. (1999) Immunity 11:699-708.
本产品仅供科研使用。不能用于人和动物治疗等其它临床诊断使用