测定意义：ATP 广泛存在于动物、植物、微生物和培养细胞中，是生物能量通货，能荷是描述细胞能量代谢状态的主要参数。
测定原理：测定ATP 含量并且计算能荷，能够反映能量代谢状态。肌酸激酶催化肌酸和ATP 反应生成磷酸肌酸，可在700nm 下用磷钼酸比色法检测磷酸肌酸含量，以此反应ATP 含量。
自备仪器和用品：分光光度计、水浴锅、可调式移液枪、1mL 石英比色皿、研钵和蒸馏水。
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