ZNF144,锌指蛋白144抗体

产品编号HZ-9673R
英文名称ZNF144
中文名称锌指蛋白144抗体
别 名DNA binding protein Mel 18; DNA-binding protein Mel-18; Mel 18; MEL-18; MGC10545; PCGF 2; PCGF2; PCGF2_HUMAN; Polycomb group ring finger 2; Polycomb group RING finger protein 2; Ring finger protein 110; RNF 110; RNF110; Zinc finger protein 144; ZNF 144; ZNF144.
说 明 书0.1ml 0.2ml
研究领域肿瘤 细胞生物 干细胞 细胞周期蛋白 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse,
ZNF144,锌指蛋白144抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量38/60kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Mel18/ZNF144
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
ZNF144,锌指蛋白144抗体PubMedPubMed
产品介绍background:
The protein encoded by this gene contains a RING finger motif and is similar to the polycomb group (PcG) gene products. PcG gene products form complexes via protein-protein interaction and maintain the transcription repression of genes involved in embryogenesis, cell cycles, and tumorigenesis. This protein was shown to act as a negative regulator of transcription and has tumor suppressor activity. The expression of this gene was detected in various tumor cells, but is limited in neural organs in normal tissues. Knockout studies in mice suggested that this protein may negatively regulate the expression of different cytokines, chemokines, and chemokine receptors, and thus plays an important role in lymphocyte differentiation and migration, as well as in immune responses. [provided by RefSeq, Jul 2008].

Function:
Transcriptional repressor. Binds specifically to the DNA sequence 5'-GACTNGACT-3'. Has tumor suppressor activity. May play a role in control of cell proliferation and/or neural cell development. Regulates proliferation of early T progenitor cells by maintaining expression of HES1. Also plays a role in antero-posterior specification of the axial skeleton and negative regulation of the self-renewal activity of hematopoietic stem cells. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Is not functionally redundant with Bmi1; unlike Bmi1 does not stimulate the E3 ubiquitin-protein ligase activity in a reconstituted PRC1-like complex.

ZNF144,锌指蛋白144抗体Subunit:
Exists as both a monomer and homodimer. Component of a PRC1-like complex. Interacts WITH CBX8, RING1 AND RNF2.

Subcellular Location:
Nucleus.

Tissue Specificity:
Detected in all tissues examined with high expression found in placenta lung and kidney and low expression, in liver, pancreas and skeletal muscle.

Post-translational modifications:
Phosphorylated. Homodimer formation is regulated by phosphorylation state with unphosphorylated protein forming homodimers.

Similarity:
Contains 1 RING-type zinc finger.

Database links:
Entrez Gene: 7703 Human
Entrez Gene: 22658 Mouse
Omim: 600346 Human
SwissProt: P35227 Human
SwissProt: P23798 Mouse
Unigene: 371617 Human
Unigene: 713595 Human
Unigene: 2418 Mouse

ZNF144,锌指蛋白144抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.