BUTYL-S SEPHAROSE 6 FF
产品名称： BUTYL-S SEPHAROSE 6 FF
英文名称： BUTYL-S SEPHAROSE 6 FF
产品编号： 17-0978-10
产品价格： 0
产品产地： 美国
品牌商标： GE
更新时间： null
使用范围： null
- 联系人 :
- 地址 : 上海市宝山区友谊路1506号东智广场1109室
- 邮编 : 201900
- 所在区域 : 上海
- 电话 : 138****5983 点击查看
- 传真 : 点击查看
- 邮箱 : 342786402@qq.com
| 17-0978-02 | BUTYL-S SEPHAROSE 6 FF, 200 ML |
| 17-0978-10 | BUTYL-S SEPHAROSE 6 FF, 25ML |
| 17-0980-01 | BUTYL SEPHAROSE 4 FAST FLOW 20 |
| 17-0980-10 | BUTYL SEPHAROSE FAST FLOW, 25 |
| 17-1014-01 | Q SEPHAROSE HIGH PERF. 75 ML |
| 17-1082-01 | PHENYL SEPHAROSE HIGH PERF. 75 |
| 17-1087-01 | SP SEPHAROSE HIGH PERF. 75 ML |
| 17-1273-01 | SOURCE 30S, 50 ML |
| 17-1275-01 | SOURCE 30Q, 50 ML |
| 17-1287-01 | ANX SEPH 4 FF HIGH SUB 500ML |
| 17-1287-10 | ANX SEPHAROSE FF HIGHSUB 25 ML |
| 17-5072-01 | Q SEPHAROSE XL, 300 ML |
| 17-5073-01 | SP SEPHAROSE XL, 300 ML |
| 17-5316-02 | Capto Q 100 ml |
| 17-5316-10 | CAPTO Q, 25 ML |
| 17-5317-02 | Capto MMC 100ml |
| 17-5317-10 | CAPTO MMC, 25ML |
| 17-5432-01 | Butyl Sepharose HP, 25 ml |
| 17-5432-02 | Butyl Sepharose HP, 200ml |
| 17-5437-01 | Q SEPHAROSE XL VIRUS LIC 300ML |
| 17-5437-10 | Q SEPHAROSE XL VIRUS LIC 25 ML |
| 17-5441-01 | CAPTO S, 100 ML |
| 17-5441-10 | CAPTO S, 25 ML |
| 17-5443-01 | CAPTO DEAE, 100 ML |
| 17-5443-10 | CAPTO DEAE, 25 ML |
| 17-5444-10 | CAPTO adhere 25 ML |
Butyl-S Sepharose™ 6 Fast Flow
- Butyl-S Sepharose 6 Fast Flow* designed for purification of relatively strong hydrophobic molecules at low salt concentrations.
- Useful for removal of strong hydrophobic contaminants, such as lipids, lipoproteins, pigments etc from biological samples.
- Used successfully for large-scale purification of recombinant Hepatitis B virus surface antigen (rec-HBsAg) from CHO cells.
- Robust production with high reproducibility.
- The least hydrophobic media in our range.
- Available prepacked as HiTrap Butyl-S FF for convenient purification and/or screening of optimal binding conditions
)
Butyl-S Sepharose™ 6 Fast Flow
Technical Information
Butyl-S Sepharose 6 Fast Flow is the least hydrophobic medium in the Fast Flow series for both research and industrial applications.
. This arrangement has the advantage that the weaker HIC adsorbent (Butyl-S Sepharose 6 Fast Flow) effectively bound the most hydrophobic plant pigments without binding the miraculin. This prevented damage to the capture column (Phenyl Sepharose 6 Fast Flow) by the plant pigments, which bind to it so strongly that regeneration is difficult, if not impossible.))
Elution profile obtained after HIC of a concentrated crude extract of miraculin on a tandem column of Butyl-S Sepharose 6 Fast Flow coupled in series to Phenyl Sepharose 6 Fast Flow (see licensing information regarding purification of miraculin using Butyl-S Sepharose 6 Fast Flow at back of catalog). This arrangement has the advantage that the weaker HIC adsorbent (Butyl-S Sepharose 6 Fast Flow) effectively bound the most hydrophobic plant pigments without binding the miraculin. This prevented damage to the capture column (Phenyl Sepharose 6 Fast Flow) by the plant pigments, which bind to it so strongly that regeneration is difficult, if not impossible.
| TECHNICAL SPECIFICATIONS | |
| Ligand | Butyl-S |
| Ligand density | 10 μmol/ml medium |
| Matrix | Highly cross-linked agarose, 6% |
| Average particle size | 90 μm |
| Pressure/flow spec. | 200-400 cm/h, 1 bar, XK 50/60 column, bed height 25 cm |
| pH stability | 2-14 (short term), 3-13 (long term) |
| Chemical stability |
Stable in commonly used aqueous buffers: 1 mM HCl, 1 M NaOH, 30% isopropanol, 50% ethylene glycol, 70% ethanol, 3 M ammonium sulfate, 6 M guanidine hydrochloride, 8 M urea. |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 30°C |
| Autoclavable | In 0.05 M sodium phosphate, pH 7.0 at 120°C for 20 min. |