Annexin I,膜粘连蛋白 I抗体

产品编号HZ-1171R
英文名称Annexin I
中文名称膜粘连蛋白 I抗体
别 名Annexin I; AnnexinI; Annexin I (lipocortin I); Annexin 1; Annexin-1; Annexin1; AnnexinA1; Annexin A1; AnnexinI; ANX 1; ANX A1; ANX1; ANXA 1; ANXA1; ANXA1 protein; Calpactin 2; Calpactin II; CalpactinII; Chromobindin 9; Chromobindin9; HGNC:533; Lipocortin 1; Lipocortin I; ANXA1_HUMAN; Chromobindin-9; Lipocortin 1; Lipocortin I; Lipocortin1; LipocortinI; Lipocortin1; LipocortinI; LPC 1; LPC1; p35; Phospholipase A2 inhibitory protein.
规格价格0.1ml 0.2ml
说 明 书0.1ml 0.2ml
Annexin I,膜粘连蛋白 I抗体研究领域细胞生物 细胞骨架
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Dog, Pig, Cow, Horse,
产品应用ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量39kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Annexin I C-terminus
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Annexin I,膜粘连蛋白 I抗体PubMedPubMed
产品介绍background:
This gene encodes a protein that belongs to a family of Ca(2+) dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti inflammatory activity.

Function:
Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.

Subunit:
Homodimer in placenta (20%); linked by transglutamylation. Interacts with DYSF (By similarity).

Subcellular Location:
Nucleus (By similarity). Cytoplasm (By similarity). Cell projection, cilium (By similarity). Basolateral cell membrane (By similarity). Note=Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages (By similarity).

Post-translational modifications:
Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity.

Similarity:
Belongs to the annexin family.
Contains 4 annexin repeats.

Gene ID:
301

Annexin I,膜粘连蛋白 I抗体Database links:
Entrez Gene: 301 Human
Entrez Gene: 16952 Mouse
Entrez Gene: 25380 Rat
Omim: 151690 Human
SwissProt: P04083 Human
SwissProt: P10107 Mouse
SwissProt: P07150 Rat
Unigene: 494173 Human
Unigene: 248360 Mouse
Unigene: 1792 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Annexin 1是一种分子量为35～36KD的Ca2+依赖性磷脂结合蛋白，能与PS高亲和力特异性结合。
钙依赖性磷脂结合蛋白其意义：检测早期凋亡的细胞，同时可区分活细胞和坏死细胞磷脂酰丝氨酸（Phosphatidylserine，PS）正常位于细胞膜的内侧，但在细胞凋亡的早期，PS可从细胞膜的内侧翻转到细胞膜的表面，暴露在细胞外环境中.

产品图片

Tissue/cell: human rectal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Annexin I Polyclonal Antibody, Unconjugated(bs-1171R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining