连环蛋白β1(CTNNβ1)重组蛋白

YB021Ra012
Recombinant Catenin Beta 1 (CTNNb1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
连环蛋白β1(CTNNβ1)重组蛋白[ PROPERTIES ]
Residues: Cys439~Ser675 (Accession # P35222),
with N-terminal His-Tag.
Host: E. coli
Subcellular Location: Cytoplasm. Nucleus.
Cytoskeleton. Cell junction, adherens junction.
Cell membrane. centrosome. spindle pole.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
连环蛋白β1(CTNNβ1)重组蛋白pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 7.0
Predicted Molecular Mass: 27.7kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
连环蛋白β1(CTNNβ1)重组蛋白[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-CQ VGGIEALVRT VLRAGDREDI TEPAICALRH LTSRHQEAEM
AQNAVRLHYG LPVVVKLLHP PSHWPLIKAT VGLIRNLALC PANHAPLREQ GAIPRLVQLL
VRAHQDTQRR TSMGGTQQQF VEGVRMEEIV EGCTGALHIL ARDVHNRIVI RGLNTIPLFV
QLLYSPIENI QRVAAGVLCE LAQDKEAAEA IEAEGATAPL TELLHSRNEG VATYAAAVLF
RMSEDKPQDY KKRLS