Thioredoxin抗体-抗体-抗体-生物在线
上海信裕生物科技有限公司
Thioredoxin抗体

Thioredoxin抗体

商家询价

产品名称： Thioredoxin抗体

英文名称： ThioredoxinAntibody

产品编号： XY14999-1

产品价格： null

产品产地： 中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

上海信裕生物科技有限公司
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  • 邮编 : 200612
  • 所在区域 : 上海
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 THIOREDOXIN抗体Applications

In-house applications ELISA, IHC, IF, FC, IP, WB     Application not tested? Please see our Guarantee.
Published applications CoIP, IHC, WB Publications 5 citations –view here
Species specificity Human,Mouse,Rat; other species not tested.
Published Species Human, mouse, rat
Images
Prev12NextView more validation data
  • 14999-1-AP;human liverImmunohistochemical of paraffin-embedded human liver using 14999-1-AP(TXN antibody) at dilution of 1:100 (under 10x lens)
  • 14999-1-AP;human liverImmunohistochemical of paraffin-embedded human liver using 14999-1-AP(TXN antibody) at dilution of 1:100 (under 40x lens)
  • 14999-1-AP;HelaImmunofluorescent analysis of Hela cells, using TXN antibody 14999-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
  • 14999-1-AP;Jurkat cellsJurkat cells were subjected to SDS PAGE followed by western blot with 14999-1-AP( TXN Antibody) at dilution of 1:2000
  • 14999-1-AP;Jurkat cellsIP Result of anti-TXN (IP:14999-1-AP, 4ug; Detection:14999-1-AP 1:300) with Jurkat cells lysate 2000ug.
  • 14999-1-AP;HeLa cells1X10^6 HeLa cells were stained with 0.2ug TXN antibody (14999-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
Positive WB detected in Jurkat cells, A2780 cells, HeLa cells, human brain tissue, human liver tissue, mouse liver tissue, rat brain tissue
Positive IP detected in Jurkat cells
Positive IHC detected in Human liver tissue, human cervical cancer tissue, human skeletal muscle tissue
Positive IF detected in Hela cells
Positive FC detected in HeLa cells
Recommended dilution WB : 1:1000-1:10000
IP : 1:200-1:1000
IHC : 1:50-1:200
IF : 1:20-1:200
 THIOREDOXIN抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information THIOREDOXIN抗体
Immunogen Thioredoxin fusion protein ag6989 Full name Thioredoxin
Calculated molecular
weight		 12 kDa Observed molecular 
weight		 12 kDa
GenBank accession 
number		 BC003377 Gene ID (NCBI) 7295
Gene symbol TXN Synonyms ADF, ATL derived factor, DKFZp686B1993, SASP, Thioredoxin, TRDX, TRX, TRX1, TXN
Background

TXN, TRDX, TRX, TRX1, ADF and SASP, belongs to the thioredoxin family. It participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity. THIOREDOXIN抗体

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