蛋白二硫化物异构酶A6(PDIA6)重组蛋白

YB776Hu012
Recombinant Protein Disulfide Isomerase A6 (PDIA6)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
 蛋白二硫化物异构酶A6(PDIA6)重组蛋白[ PROPERTIES ]
Residues: Leu20~Leu440
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q15084
Host: E. coli
Subcellular Location: Endoplasmic reticulum lumen.
Cell membrane. Melanosome.
Purity: >90%
Endotoxin Level: <1.0EU per 1μg
 蛋白二硫化物异构酶A6(PDIA6)重组蛋白(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 5.0
Predicted Molecular Mass: 49.9kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
 蛋白二硫化物异构酶A6(PDIA6)重组蛋白than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
L YSSSDDVIEL TPSNFNREVI QSDSLWLVEF YAPWCGHCQR LTPEWKKAAT ALKDVVKVGA
VDADKHHSLG GQYGVQGFPT IKIFGSNKNR PEDYQGGRTG EAIVDAALSA LRQLVKDRLG
GRSG GYS SGK QG RS DSS SK K DVIE LT D DS F DK NV L DS E DV WMVE FYAPW C
GHCKNLEPEW AAAASEVKEQ TKGKVKLAAV DATVNQVLAS RYGIRGFPTI KIFQKGESPV
DYDGGRTRSD IVSRALDLFS DNAPPPELLE IINEDIAKRT CEEHQLCVVA VLPHILDTGA
AGRNSYLEVL LKLADKYKKK MWGWLWTEAG AQSELETALG IGGFGYPAMA AINARKMKFA
LLKGSFSEQG INEFLRELSF GRGSTAPVGG GAFPTIVERE PWDGRDGELP VEDDIDLSDV
ELDDLGKDEL