IL32 (Human) Recombinant Protein
产品名称： IL32 (Human) Recombinant Protein
英文名称： IL32 (Human) Recombinant Protein
产品编号： P4565
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
- 联系人 :
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- 邮编 : 11493
- 所在区域 : 台湾
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- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Human IL32 (AAS80146) recombinant protein expressed in Escherichia coli.
- Sequence:
- MCFPKVLSDDMKKLKARMHQAIERFYDKMQNAESGRGQVMSSLAELEDDFKEGYLETVAAYYEEQHPELTPLLEKERDGLRCRGNRSPVPDVEDPATEEPGESFCDKSYGAPRGDKEELTPQKCSEPQSSK
- Theoretical MW (kDa):
- 14.9
- Form:
- Lyophilized
- Preparation Method:
- Escherichia coli expression system
- Endotoxin Level:
- < 0.01 ng/ug or < 0.1 EU/ug.
- Activity:
- The activity is determined by the dose-dependent induction of TNF alpha production from human PBMCs. The expected ED50 for this effect is 101-152 ng/mL.
- Storage Buffer:
- Lyophilized with 50 mM Na2PO4, pH 7.5.
- Storage Instruction:
- Store at -20°C on dry atmosphere.
After reconstitution with sterilized water, store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- 1 ug/lane in 4-20% Tris-Glycine gel Stained with Coomassie Blue
Lane 1: non-reducing conditions
Lane 2: reducing conditions
- Note:
- Result of activity analysis
-
Human PBMCs were cultured with 0 to 1000 ng/mL human IL32 in serum free media. Human TNF alpha production was measured and the linear portion of the curve was us used to calculate the ED50.
- Application Image
- Functional Study
- SDS-PAGE
- Entrez GeneID:
- 9235
- Protein Accession#:
- AAS80146
- Gene Name:
- IL32
- Gene Alias:
- IL-32alpha,IL-32beta,IL-32delta,IL-32gamma,NK4,TAIF,TAIFa,TAIFb,TAIFc,TAIFd
- Gene Description:
- interleukin 32
- Omim ID:
- 606001
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFalpha from macrophage cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
- Other Designations:
- natural killer cell transcript 4,natural killer cells protein 4,tumor necrosis factor alpha-inducing factor