HA tag Mouse mAb is part of the series of Tag antibodies, the excellent quality in the research. HA tag antibody is a highly sensitive and affinity monoclonal antibody applicable to HA-tagged fusion protein detection. HA tag antibody can detect HA tags in internal, C-terminal, or N-terminal recombinant proteins. 

Product Name: HA-tag mouse monoclonal antibody 

Clone Number: 2B11 

Isotype: IgG1 

Immunogen: A synthetic peptide YPYDVPDYA coupled to KLH. 

Tested applications and dilutions: WB 1:5000-2000     IP 1:200      IF 1:1000 

Produce anti- HAantibody affinity column purify HArecombinant proteins 

Form: Liquid 

Concentration: 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol 

Storage: HAantibody is stable for 1 month at 2-8°C. For long term storage, Aliquot and store at -20°C or below. 

Avoid repeated freezing and thawing cycles.

A: HEK 293 cell lysate without transfected 

B: HEK 293 cell lysate transfected with HA-tag protein 

C: IP (HEK 293 cell without transfected + anti- HA mAb 4℃ overnight) 

D: IP (PBS + anti- HA mAb 4℃ overnight) 

E: IP (HEK293 transfected+protein G+anti- HA mAb 4℃ overnight) 

F: IP (HEK293 transfected+RIPA+Protein G 4℃ overnight) 

G: Multi Tag Recombinant protein (expressed in E.coli) 

TP=Target Protein HC=Heavy chain LC=Light Chain 

IF analysis of 293T cells transfected with a HA-tagged protein, 

using easybio HA-Tag (2B11) Mouse mAb at a 1:2000 dilution (blue