GFP monoclonal antibody
产品名称： GFP monoclonal antibody
英文名称： GFP monoclonal antibody
产品编号： MAB2003
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against full length recombinant GFP.
- Immunogen:
- Recombinant GST fusion protein corresponding to full length jellyfish Aequorea victoria GFP.
- Host:
- Mouse
- Reactivity:
- Jellyfishes
- Specificity:
- Reactivity is observed against wild-type and recombinant forms of GFP. No reaction is seen against RFP.
- Form:
- Liquid
- Isotype:
- IgG, gamma 1
- Storage Buffer:
- In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
- Recommend Usage:
- ELISA (1:200000)
Western Blot (1:3000-1:30000)
Immunohistochemistry (1:1000-1:5000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Numb/Numbl-opo antagonism controls retinal epithelium morphogenesis by regulating integrin endocytosis.
Bogdanovic O, Delfino-Machin M, Nicolas-Perez M, Gavilan MP, Gago-Rodrigues I, Fernandez-Minan A, Lillo C, Rios RM, Wittbrodt J, Martinez-Morales JR.Dev Cell. 2012 Oct 16;23(4):782-95. doi: 10.1016/j.devcel.2012.09.004. Epub 2012 Oct 4.
- Applications
- Western Blot (Transfected lysate)
- Western blot of GFP recombinant protein detected with GFP monoclonal antibody (Cat # MAB2003).
GFP recombinant protein was expressed in HeLa cells, where 50 ng (Lane 1), 100 ng (Lane 2) and 500 ng (Lane 3) of lysate were loaded per lane.
GFP monoclonal antibody (Cat # MAB2003) detects a 27 KDa band corresponding to the epitope tag GFP.
The cell lysates were prepared in a RIPA buffer containing 200 mM NaCl.
A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE.
The protein was transferred to nitrocellulose using standard methods.
After blocking with 5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted to 1.0 ug/mL for 1 h at room temperature followed by washes and reaction with a 1 : 2,500 dilution of IRDye® 800 conjugated Goat-a-Mouse IgG [H&L] MX10.
IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.