Product: Anti-IL-1RAcP (C2), Polyclonal
Cat. No: PC-045 (100 μg)
Background:
The pro-inflammatory cytokine IL-1 induced
cellular response requires two subunits of its
receptor, IL-1 receptor I (IL-1RI) and IL-1
receptor accessory protein (IL-1RAcP). IL-
1RAcP forms a complex with IL-1RI in response
to IL-1 treatment. The IL-1 receptor-associated
kinase (IRAK), which mediates activation of NF-
kb inducing kinase (NIK) and of NF-kb, recruits
to the IL-1R complex through IL-1RacP. IL-1
activation of stress-activated protein kinase and
of acid sphingomyelinase also requires IL-
1RacP. Like IL-1RI, IL-1RAcP subunit is
essential for IL-1 mediated cellular response. IL-
1RAcP is expressed in many tissues.
Specificity:
Recognizes IL-1RacP(C2). Antibody has no
cross reactivity to other members in the IL-1
receptor family.
Positive control: Whole cell lysate from HeLa
cells. An approximately 66 kDa band should be
detected.
Species Reactivity:
Human, mouse and rat, other species not tested.
Ig Isotype:
Rabbit IgG
Immunogen:
A peptide corresponding to amino acids 525 to
540 of human IL-1RAcP, which is identical to
those of mouse and rat origins.
Format:
Immunoaffinity chromatography purified IgG,
100 μg in 200 μl of PBS containing 0.02
sodium azide. Concentration: 500 μg/ml.
Storage and Stability:
Stable for at least one year when stored at 4°C.
Applications and Suggested Dilutions:

Western blot: Suggested dilution: 1:1000 to
1:2000.
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.