PAPOL A+B+G,多聚合酶α/β/γ抗体

产品编号HZ-9435R
英文名称PAPOL A+B+G
中文名称多聚合酶α/β/γ抗体
别 名PAPOL A/B/G; PAP alpha/beta/gamma; PapIII; MGC5378; PAP alpha; PAP beta; PAP gamma; PAP; PAP-alpha; PAP-beta; PAP-gamma; PAPOLA; PAPOLB; PAPOLG; PAPOA_HUMAN; PAPOB_HUMAN; PAPOG_HUMAN; Plap; poly(A) polymerase alpha; Polynucleotide adenylyltransferase alpha; poly(A) polymerase beta (testis specific); poly(A) polymerase beta; polynucleotide adenylyltransferase beta; TPAP; Testis-specific poly(A) polymerase.
说 明 书0.1ml 0.2ml
研究领域心血管 细胞生物 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Cow,
PAPOL A+B+G,多聚合酶α/β/γ抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量70-82kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human PAPOLA/PAPOLB/PAPOLG
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PAPOL A+B+G,多聚合酶α/β/γ抗体PubMedPubMed
产品介绍background:
Polyadenylation of the 3-prime ends of eukaryotic mRNAs is a key event that takes place in the nucleus during maturation of mRNA. The reaction includes endoribonucleolytic cleavage of the pre-RNA at the poly(A) site that leads to synthesis of the poly(A) tail at the 3-prime end of the upstream cleavage product. The poly(A) polymerase (PAP) is required The adenosine addition reaction depends on poly(A) polymerase (PAP) activity. The testis express PAP-beta (TPAP) in the cytoplasm of spermatogenic cells. The adenosine addition function of PAP-beta plays a critical role in male germ cell production. PAP-beta-deficient transgenic mice display impaired expression of haploid-specific genes that are necessary for spermatogenesis. The intronless gene encoding human PAP-beta maps to chromosome 7p22.3.

Function:
Polymerase that creates the 3'-poly(A) tail of mRNA's. Also required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. May acquire specificity through interaction with a cleavage and polyadenylation specificity factor (CPSF) at its C-terminus.

Subunit:
Monomer. Found in a complex with CPSF1, FIP1L1 and PAPOLA. Interacts with FIP1L1 (By similarity). Interacts with NUDT21; the interaction is diminished by acetylation. Interacts with KPNB1; the interaction promotes PAP nuclear import and is inhibited by acetylation of PAP.

Subcellular Location:
Cytoplasm. Nucleus.

Post-translational modifications:
Polysumoylated. Varying sumolyation depending on tissue- and cell-type. Highly sumoylated in bladder and NIH 3T3 cells. Sumoylation is required for nuclear localization and enhances PAP stability. Desumoylated by SENP1. Inhibits polymerase activity.
Hyperphosphorylation on multiple CDK2 consensus and non-consensus sites in the C-terminal Ser/Thr-rich region represses PAP activity in late M-phase. Phosphorylation/dephosphorylation may regulate the interaction between PAP and CPSF.
Acetylated in the C-terminus. Acetylation decreases interaction with NUDT21 and KPNB1, and inhibits nuclear localization through inhibiting binding to the importin alpha/beta complex.

PAPOL A+B+G,多聚合酶α/β/γ抗体Similarity:
Belongs to the poly(A) polymerase family.

Gene ID:
10914

Database links:
Entrez Gene: 10914 Human
Entrez Gene: 18789 Mouse
Entrez Gene: 314417 Rat
Omim: 605553 Human
SwissProt: P51003 Human
SwissProt: Q61183 Mouse
Unigene: 253726 Human
Unigene: 255877 Mouse

PAPOL A+B+G,多聚合酶α/β/γ抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.