PCDHA11,原钙粘蛋白11抗体

产品编号HZ-11124R
英文名称PCDHA11
中文名称原钙粘蛋白11抗体
别 名PCDHA 11; PCDHA-11; Protocadherin alpha-11; Protocadherin alpha 11; PCDAB_HUMAN.
说 明 书0.1ml 0.2ml
研究领域神经生物学 信号转导 细胞骨架
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Cow, Horse, Sheep,
PCDHA11,原钙粘蛋白11抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量100kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human PCDHA11
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PCDHA11,原钙粘蛋白11抗体PubMedPubMed
产品介绍background:
Protocadherins are a large family of cadherin-like cell adhesion proteins that are involved in the establishment and maintenance of neuronal connections in the brain. There are three protocadherin (PCDH) gene clusters, designated alpha, beta and gamma, all of which contain multiple tandemly arranged genes. The protein products of PCDH-alpha genes interact with Integrin ∫1 to promote cell adhesion and form oligomers with PCDH-gamma proteins at specific membrane sites. PCDHA11 is a 949 amino acid single-pass transmembrane protein that contains six cadherin domains and functions as a potential calcium-dependent cell-adhesion protein, possibly playing a role in the creation and maintenance of neuronal connections. There are two isoforms of PCDHA11 that are produced as a result of alternative splicing events.

Function:
Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.

Subcellular Location:
Cell membrane; Single-pass type I membrane protein (By similarity).

Similarity:
Contains 6 cadherin domains.

Database links:
UniProtKB/Swiss-Prot: Q9Y5I1.1

PCDHA11,原钙粘蛋白11抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-PCDHA11 Polyclonal Antibody, Unconjugated(HZ-11124R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining