KCC2,神经细胞钾氯离子转运蛋白抗体

产品编号HZ-8517R
英文名称KCC2
中文名称神经细胞钾氯离子转运蛋白抗体
别 名Electroneutral potassium chloride cotransporter 2; Electroneutral potassium-chloride cotransporter 2; Erythroid K Cl cotransporter 2; Erythroid K-Cl cotransporter 2; Furosemide sensitive K Cl cotransporter; hKCC2; K-Cl cotransporter 2; KCC 2; KCC2; KIAA1176; Neuronal K Cl cotransporter; Neuronal K-Cl cotransporter; S12A5; S12A5_HUMAN; SLC12A5; Solute carrier family 12 (potassium chloride transporter) member 5; Solute carrier family 12 member 5.
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学 神经生物学 通道蛋白
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse,
KCC2,神经细胞钾氯离子转运蛋白抗体产品应用WB=1:100-500 IHC-P=1:100-500 IHC-F=1:100-500 ICC= IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量123kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human KCC2/SLC12A5
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
KCC2,神经细胞钾氯离子转运蛋白抗体PubMedPubMed
产品介绍background:
K-Cl cotransporters are proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The protein encoded by this gene is an integral membrane K-Cl cotransporter that can function in either a net efflux or influx pathway, depending on the chemical concentration gradients of potassium and chloride. The encoded protein can act as a homomultimer, or as a heteromultimer with other K-Cl cotransporters, to maintain chloride homeostasis in neurons. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Sep 2008]

Function:
Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons.

Subunit:
Homomultimer and heteromultimer with other K-Cl cotransporters. Interacts with AP2A1.

Subcellular Location:
Membrane; Multi-pass membrane protein.

Tissue Specificity:
Brain specific. Detected in neuronal cells.

Similarity:
Belongs to the SLC12A transporter family.

Gene ID:
57468

KCC2,神经细胞钾氯离子转运蛋白抗体Database links:
Entrez Gene: 57468 Human
Entrez Gene: 57138 Mouse
Entrez Gene: 171373 Rat
Omim: 606726 Human
SwissProt: Q9H2X9 Human
SwissProt: Q91V14 Mouse
SwissProt: Q63633 Rat
Unigene: 21413 Human
Unigene: 252987 Mouse
Unigene: 10513 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-KCC2/SLC12A5 Polyclonal Antibody, Unconjugated(HZ-8517R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-KCC2/SLC12A5 Polyclonal Antibody, Unconjugated(HZ-8517R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(HZ-0295G-Cy3, red)used at 1:200 dilution for 40 minutes at 37°C.  Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-KCC2 Polyclonal Antibody, Unconjugated(HZ-8517R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining