血管内皮生长因子165(VEGF165)重组蛋白

YB696Hu014
Vascular Vascular Vascular Vascular Endothelial Endothelial Endothelial Endothelial Growth Factor 165 (VEGF165) (VEGF165) (VEGF165) (VEGF165)
Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human)
Instruction Instruction Instruction Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
血管内皮生长因子165(VEGF165)重组蛋白NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
5th Edition (Revised in January, 2013)
[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]
Protein Protein Protein Protein Names: Vascular Endothelial Growth Factor
165
Synonyms: Synonyms: Synonyms: Synonyms: VEGF165
Species: Species: Species: Species: Human
Size: 10µg
血管内皮生长因子165(VEGF165)重组蛋白Source: Source: Source: Source: Escherichia coli-derived
[ PROPERTIES PROPERTIES PROPERTIES PROPERTIES ]
Residues: Residues: Residues: Residues: Pro28~Asn141 (Accession # P15692), with
N-terminal His-Tag. Grade & Purity: Purity: Purity: Purity: >95%, 16kDa as determined by
SDS-PAGE reducing conditions.
Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS, pH
7.4, containing 5% sucrose, 0.01% sarcosyl.
Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by
the LAL method).
Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 14.7kDa
Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 5.9
[ PREPARATION PREPARATION PREPARATION PREPARATION ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
血管内皮生长因子165(VEGF165)重组蛋白[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF- PMAEGGGQNH HEVVKFMDVY QRSYCHPIET LVDIFQEYPD
EIEYIFKPSC VPLMRCGGCC NDEGLECVPT EESNITMQIM RIKPHQGQHI GEMSFLQHNK
CECRPKKDRA RQEN
[ REFERENCES REFERENCES REFERENCES REFERENCES ]
1. Bates D.O., et al. (2002) Cancer Res. 62:4123-4131(2002). 2. Woolard J., et al. (2004) Cancer Res. 64:7822-7835(2004). 3. Murata H., et al. Submitted (DEC-1998) to the EMBL/GenBank/DDBJ
databases. 4. Shan Z.X., et al. Submitted (FEB-2002) to the EMBL/GenBank/DDBJ
databases.