MMP12 polyclonal antibody
产品名称： MMP12 polyclonal antibody
英文名称： MMP12 polyclonal antibody
产品编号： PAB4781
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of MMP12.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human MMP12.
- Host:
- Rabbit
- Reactivity:
- Human, Mouse
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Crystal structure of human macrophage elastase (MMP-12) in complex with a hydroxamic acid inhibitor.
Nar H, Werle K, Bauer MM, Dollinger H, Jung B.J Mol Biol. 2001 Sep 28;312(4):743-51.
- 2.
- Substrate specificity determinants of human macrophage elastase (MMP-12) based on the 1.1 A crystal structure.
Lang R, Kocourek A, Braun M, Tschesche H, Huber R, Bode W, Maskos K.J Mol Biol. 2001 Sep 28;312(4):731-42.
- 3.
- Hydrolysis of a broad spectrum of extracellular matrix proteins by human macrophage elastase.
Gronski TJ Jr, Martin RL, Kobayashi DK, Walsh BC, Holman MC, Huber M, Van Wart HE, Shapiro SD.J Biol Chem. 1997 May 2;272(18):12189-94.
- Applications
- Western Blot (Tissue lysate)
- Western blot analysis of MMP12 polyclonal antibody (Cat # PAB4781) in mouse kidney tissue lysate (35 ug/lane). MMP12 (arrow) was detected using the purified polyclonal antibody (1 : 240 dilution).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MMP12 polyclonal antibody (Cat # PAB4781) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
- Entrez GeneID:
- 4321
- Protein Accession#:
- MM12_HUMAN
- Gene Name:
- MMP12
- Gene Alias:
- HME,MGC138506,MME
- Gene Description:
- matrix metallopeptidase 12 (macrophage elastase)
- Omim ID:
- 601046
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq
- Other Designations:
- macrophage elastase,macrophage metalloelastase,matrix metalloproteinase 12,matrix metalloproteinase 12 (macrophage elastase)
- Related Disease
- Acute Disease
- Adenocarcinoma
- alpha 1-Antitrypsin Deficiency
- Alpha-1-antitrypsin deficiency
- Amyotrophic Lateral Sclerosis
- Amyotrophic lateral sclerosis
- Anoxia
- Aortic Aneurysm, Abdominal
- Arthritis, Rheumatoid
- Asthma
- Asthma
- Atherosclerosis
- Breast cancer
- Breast Neoplasms
- Carcinoma, Non-Small-Cell Lung
- Carcinoma, Squamous Cell
- Cardiovascular Diseases
- Carotid Artery, Internal, Dissection
- Chorioamnionitis