Deltex 1,DTX1抗体

产品编号HZ-0399R
英文名称Deltex 1
中文名称DTX1抗体
别 名Deltex protein 1; Deltex; Deltex-1; Deltex1; Dtx1; FXI-T1; Fxit 1; Fxit1; mDTX1; DTX1_HUMAN.
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学 发育生物学 神经生物学 干细胞 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Cow,
Deltex 1,DTX1抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量67kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Deltex-1
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Deltex 1,DTX1抗体PubMedPubMed
产品介绍background:
Deltex is a RING finger ubiquitin ligase which is conserved from Drosophila to Humans and is a regulator of Notch signaling pathway. Deltex regulates both B-cell lineage and splenic marginal-zone B-cell commitment. Deltex is implicated in neurogenesis, lymphogenesis and myogenesis as well as marginal zone B cell differentiation.

Function:
Functions as an ubiquitin ligase protein in vivo, mediating ubiquitination and promoting degradation of MEKK1, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity (By similarity). Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Mainly acts as a positive regulator of Notch, but it also acts as a negative regulator, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Involved in neurogenesis, lymphogenesis and myogenesis, and may also be involved in MZB (Marginal zone B) cell differentiation. Promotes B-cell development at the expense of T-cell development, suggesting that it can antagonize NOTCH1.

Subunit:
Homodimer. May form a heterodimer with other members of the Deltex family. Interacts with NOTCH1 via its N-terminus region and EIF3F, the interaction is required for NOTCH1 deubiquitination. Interacts with EP300. Forms a heterodimer with BBAP; the heterodimerization leading to an increase of in vitro ubiquitin ligase activity. Interacts with ITCH.

Subcellular Location:
Cytoplasm. Nucleus. Note=Predominantly cytoplasmic. Associates with endocytic vesicles. Partially nuclear.

Tissue Specificity:
Widely expressed. Strongly expressed in blood vessel. Also expressed in embryonic nervous system, pancreas, lung, adrenal gland, digestive tube and muscles. Expressed in MZB cells and developing B- and T-cells.

Post-translational modifications:
Ubiquitinated; undergoes 'Lys-29'-linked polyubiquitination catalyzed by ITCH.

Similarity:
Belongs to the Deltex family.
Contains 1 RING-type zinc finger.
Contains 2 WWE domains.

Gene ID:
1840

Database links:
Entrez Gene: 1840 Human
Entrez Gene: 14357 Mouse
Omim: 602582 Human
SwissProt: Q86Y01 Human
SwissProt: Q61010 Mouse

Deltex 1,DTX1抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Deltex1用于老年滞呆、神经退行性变的研究。

产品图片

Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Deltex 1 Polyclonal Antibody, Unconjugated(HZ-0399R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei