FITC标记的剪切和多聚腺苷酸化特异性因子4抗体
产品名称： FITC标记的剪切和多聚腺苷酸化特异性因子4抗体
英文名称： Anti-CPSF4/FITC
产品编号： HZ-2253R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围：
上海沪震实业有限公司
- 联系人 : 鲍丽雯
- 地址 : 上海市闵行区闵北路88弄1-30号第22幢AQ136室
- 邮编 : 200612
- 所在区域 : 上海
- 电话 : 139****0749 点击查看
- 传真 : 点击查看
- 邮箱 : www.shzbio.net
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Rabbit Anti-CPSF4/FITC Conjugated antibody
| FITC标记的剪切和多聚腺苷酸化特异性因子4抗体 |
| 英文名称 | Anti-CPSF4/FITC |
| 中文名称 | FITC标记的剪切和多聚腺苷酸化特异性因子4抗体 |
| 别 名 | Cleavage and polyadenylation specificity factor 30 kDa subunit; CPSF 30 kDa subunit; CPSF30; CPSF4; NAR; NEB1; No arches homolog; NS1 effector domain binding protein 1; CPSF4_RAT. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, |
| 产品应用 | not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 27kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CPSF4/CPSF30 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs. Multiple alternatively spliced transcript variants that encode different isoforms have been described for this gene. [provided by RefSeq, Jul 2008] Function: Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. CPSF4 binds RNA polymers with a preference for poly(U). Subunit: Component of the cleavage and polyadenylation specificity factor (CPSF) complex, composed of CPSF1, CPSF2, CPSF3, CPSF4 and FIP1L1. Interacts with FIP1L1. Subcellular Location: Nucleus. Similarity: Belongs to the CPSF4/YTH1 family. Contains 5 C3H1-type zinc fingers. Contains 1 CCHC-type zinc finger. Database links: Entrez Gene: 10898 Human Entrez Gene: 54188 Mouse Entrez Gene: 304277 Rat Omim: 603052 Human SwissProt: O95639 Human SwissProt: Q8BQZ5 Mouse SwissProt: Q5FVR7 Rat Unigene: 489287 Human Unigene: 196884 Mouse Unigene: 104788 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
抑制由A型流感病毒NS1蛋白引起的聚（A）包含的mRNA的核输出需要其效应域。NS1效应域功能与细胞30 kDa亚基的裂解和多聚腺苷酸化特异性因子4相互作用，这是细胞前mRNA的3’端加工机械的重要组成部分。在流感病毒感染的细胞中，NS1蛋白与解理和多腺苷化特异性因子4、30kD亚基物理上相关。NS1蛋白与体外30 kDa蛋白的结合阻止了CPSF与RNA底物的结合，并抑制了宿主前mRNA的3’端裂解和聚腺苷酸化。因此，NS1蛋白选择性地抑制细胞的核输出，而不是病毒、mRNA。多个交替剪接的转录变体，编码不同的异构体已被描述为该基因。[ RefSeq，JUL 2008 ]