SAE1 抗体-抗体-抗体-生物在线
上海信裕生物科技有限公司
SAE1 抗体

SAE1 抗体

商家询价

产品名称: SAE1 抗体

英文名称: SAE1 Antibody

产品编号: XY 10229-1-

产品价格: null

产品产地: 中国/美国

品牌商标: XYbscience

更新时间: 2023-08-17T09:55:27

使用范围: WB,ELISA,IHC-P,IHC-F,IF

上海信裕生物科技有限公司
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 SAE1 抗体Applications

In-house applications ELISA, WB, IF, IHC     Application not tested? Please see our Guarantee.
Species specificity Human,Mouse,Rat; other species not tested.
Images
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  • 10229-1-AP;A431 cellsA431 cells were subjected to SDS PAGE followed by western blot with 10229-1-AP(SAE1 antibody) at dilution of 1:300
  • 10229-1-AP;human colon cancer tissueImmunohistochemistry of paraffin-embedded human colon cancer tissue slide using 10229-1-AP( SAE1 Antibody) at dilution of 1:200 (under 10x lens).
  • 10229-1-AP;human colon cancer tissueImmunohistochemistry of paraffin-embedded human colon cancer tissue slide using 10229-1-AP( SAE1 Antibody) at dilution of 1:200 (under 40x lens).
  • 10229-1-AP;HeLa cellsImmunofluorescent analysis of HeLa cells using 10229-1-AP( SAE1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Positive WB detected in A431 cells, A549 cells, COLO 320 cells, HEK-293 cells, HeLa cells
Positive IHC detected in Human colon cancer tissue, human skin cancer tissue
Positive IF detected in HeLa cells
Recommended dilution WB : 1:200-1:1000
IHC : 1:20-1:200
IF : 1:20-1:200
 SAE1 抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information SAE1 抗体
Immunogen SAE1 fusion protein ag0275 Full name SUMO1 activating enzyme subunit 1
Calculated molecular
weight
38 kDa Observed molecular 
weight
38 kDa
GenBank accession 
number
BC000344 Gene ID (NCBI) 10055
Gene symbol SAE1 Synonyms AOS1, FLJ3091, HSPC140, SAE1, SUA1, UBLE1A
Background

SAE1(SUMO-activating enzyme subunit 1) is also named as AOS1, SUA1, UBLE1A and belongs to the ubiquitin-activating E1 family. SAE1 has a calculated molecular mass of 38 kD. It had an apparent molecular mass of 40 kD by SDS-PAGE. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins(PMID:9920803). Western blot analysis of synchronized HeLa cells detectes increased AOS1 expression as cells progressed through S phase, followed by a substantial decrease in G2 phase. Immunofluorescence analysis shows AOS1 and UBA2 distributed throughout nuclei, but they are excluded from nucleoli.(PMID:11481243). SAE1 抗体

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XY100Ra21 基质金属蛋白酶2(MMP2)单克隆抗体 Monoclonal Antibody to klrix Metalloproteinase 2 (MMP2) Rattus norvegicus (Rat)

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