甘露糖苷酶α1A类成员1(MAN1A1)重组蛋白

YB689Hu015
Recombinant Mannosidase Alpha Class 1A Member 1 (MAN1A1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
甘露糖苷酶α1A类成员1(MAN1A1)重组蛋白[ PROPERTIES ]
Residues: Ile322~Glu653
Tags: Two N-terminal Tags, His-tag and GST-tag
Accession: P33908
Host: E. coli
Subcellular Location: Golgi apparatus membrane.
Single-pass type II membrane protein.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
甘露糖苷酶α1A类成员1(MAN1A1)重组蛋白Formulation: Supplied as lyophilized form in 20mM Tris,
150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 5.8
Predicted Molecular Mass: 68.1kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
IPWALLNMK SGIGRNWPWA SGGSSILAEF GTLHLEFMHL SHLSGNPIFA EKVMNIRTVL
甘露糖苷酶α1A类成员1(MAN1A1)重组蛋白NK L EKP QG LY PNY L NP SS GQ WGQ H HV SVG G LGDSFY EY L L KAW LMS DK T D
LEAKKMYFDA VQAIETHLIR KSSSGLTYIA EWKGGLLEHK MGHLTCFAGG MFALGADAAP
EGMAQHYLEL GAEIARTCHE SYNRTFMKLG PEAFRFDGGV EAIATRQNEK YYILRPEVME
TYMYMW R LT H DPKY RKWAWE AVEA LE N HCR VNGGYSG LR D VY L LHESY DD
VQQSFFLAET LKYLYLIFSD DDLLPLEHWI FNSEAHLLPI LPKDKKEVEI REE