紧密连接蛋白1(TJP1)重组蛋白

YB262Ra012
Recombinant Tight Junction Protein 1 (TJP1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
 紧密连接蛋白1(TJP1)重组蛋白[ PROPERTIES ]
Residues: Met1~Val190
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q07157
Host: E. coli
Subcellular Location: Cell membrane;
Peripheral membrane protein; Cytoplasmic side.
Cell junction, tight junction. Gap junction.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
 紧密连接蛋白1(TJP1)重组蛋白(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 9.8
Predicted Molecular Mass: 24.7kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
 紧密连接蛋白1(TJP1)重组蛋白precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
MSARAAAAKS TAMEETAIWE QHTVTLHRAP GFGFGIAISG GRDNPHFQSG ETSIVISDVL
KGGPAEGQLQ ENDRVAMVNG VSMDNVEHAF AVQQLRKSGK NAKITIRRKK KVQIPVSRPD
PEPVSDNEED SYDEEIHDPR SGRSGVVNRR SEKIWPRDRS ASRERSLSPR SDRRSVASSQ
PAKPTKVTLV
[ REFERENCES ]
1. Willott E., et al. (1993) Proc. Natl. Acad. Sci. U.S.A. 90:7834-7838. 2. Cohen C.J., et al. (2001) Proc. Natl. Acad. Sci. U.S.A. 98:15191-15196. 3. Nielsen P.A., et al. (2002) J. Biol. Chem. 277:38272-38283. 4. Li X., et al. (2004) Neuroscience 126:611-630.