G protein alpha Inhibitor 2,G蛋白α抑制蛋白2抗体

产品编号HZ-9920R
英文名称G protein alpha Inhibitor 2
中文名称G蛋白α抑制蛋白2抗体
别 名G protein alpha Inhibitor 2; adenylate cyclase inhibiting G alpha protein; Adenylate cyclase-inhibiting G alpha protein; GIP; GNAI 2; GNAI2_HUMAN; GNAI2B; GTP binding regulatory protein Gi alpha 2 chain; Guanine nucleotide binding protein G protein alpha inhibiting activity polypeptide 2; guanine nucleotide binding protein G(i) alpha 1 subunit; guanine nucleotide binding protein G(i) alpha 2 subunit (adenylate cyclase inhibiting G alpha protein); Guanine nucleotide-binding protein G(i) subunit alpha-2; H LUCA15.1; H LUCA16.1; WUGSC:H LUCA15.1; WUGSC:H LUCA16.1.
说 明 书0.1ml 0.2ml
研究领域细胞生物 信号转导 细胞分化 G蛋白信号
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep,
G protein alpha Inhibitor 2,G蛋白α抑制蛋白2抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量40kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human G protein alpha Inhibitor 2
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
G protein alpha Inhibitor 2,G蛋白α抑制蛋白2抗体PubMedPubMed
产品介绍background:
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division.

Function:
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division.
Isoform sGi2: Regulates the cell surface density of dopamine receptors DRD2 by sequestrating them as an intracellular pool.

Subunit:
G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with GPSM1. Interacts with RGS12 and RGS14. Interacts with UNC5B.

Subcellular Location:
Cytoplasm. Cytoplasm, cytoskeleton, centrosome. Cell membrane. Note=Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody.

Similarity:
Belongs to the G-alpha family. G(i/o/t/z) subfamily.

Gene ID:
2771

Database links:
Entrez Gene: 2771 Human
Entrez Gene: 14678 Mouse
Entrez Gene: 81664 Rat
Omim: 139360 Human
SwissProt: P04899 Human
SwissProt: P08752 Mouse
SwissProt: P04897 Rat
Unigene: 77269 Human
Unigene: 196464 Mouse
Unigene: 3036 Rat

G protein alpha Inhibitor 2,G蛋白α抑制蛋白2抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-G protein alpha Inhibitor 2 Polyclonal Antibody, Unconjugated(HZ-9920R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining