IASPP抗体
Applications
In-house applications |
ELISA, IHC, IF, IP, WB Application not tested? Please see our Guarantee. |
Species specificity |
Human, Rat, Mouse; other species not tested. |
Images
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- Immunohistochemical of paraffin-embedded human breast cancer using 18590-1-AP(PPP1R13L antibody) at dilution of 1:100 (under 10x lens)
- Immunohistochemical of paraffin-embedded human breast cancer using 18590-1-AP(PPP1R13L antibody) at dilution of 1:100 (under 40x lens)
- Immunofluorescent analysis of MCF-7 cells, using PPP1R13L antibody 18590-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18590-1-AP( PPP1R13L Antibody) at dilution of 1:1000
- IP Result of anti-PPP1R13L (IP:18590-1-AP, 4ug; Detection:18590-1-AP 1:1000) with PC-3 cells lysate 1040ug.
|
Positive WB detected in |
NIH/3T3 cells, Apoptosised HeLa cells, C6 cells, MCF7 cells, PC-3 cells |
Positive IP detected in |
PC-3 cells |
Positive IHC detected in |
Human breast cancer tissue |
Positive IF detected in |
MCF-7 cells |
Recommended dilution |
WB : 1:500-1:5000
IP : 1:500-1:5000
IHC : 1:20-1:200
IF : 1:10-1:100 |
IASPP抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source |
Rabbit |
Purification method |
Antigen affinity purification |
Isotype |
IgG |
Storage |
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
Immunogen |
IASPP fusion protein ag13273 |
Full name |
Protein phosphatase 1, regulatory (inhibitor) subunit 13 like |
Calculated molecular
weight |
89 kDa |
Observed molecular
weight |
110 kDa |
GenBank accession
number |
BC064913 |
Gene ID (NCBI) |
10848 |
Gene symbol |
PPP1R13L |
Synonyms |
IASPP, Inhibitor of ASPP protein, NKIP1, PPP1R13B like protein, PPP1R13BL, PPP1R13L, Protein iASPP, RAI, RelA associated inhibitor |
Background IASPP抗体
Inhibitor of apoptosis-stimulating protein of p53 (iASPP), encoded by PPP1R13L gene, is often overexpressed in human cancers. The ASPP family includes three members, namely ASPP1, ASPP2, and iASPP, which are specific regulators of p53-, p63-, and p73-mediated apoptosis. ASPP1 and ASPP2 enhance the apoptotic function of p53, whereas iASPP specifically inhibits p53- mediated apoptosis. Overexpression of iASPP is associated with resistance to cisplatin-induced apoptosis and rediation therapy. iASPP plays a pivotal role in regulating cancer cell proliferation and tumor progression. This antibody could both recognize unphosphorylated and phosphorylated iASPP. IASPP抗体
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