The Microorganism Direct PCR Kit contains all of the reagents required to rapidly extract and amplify genomic DNA from desiccative Microorganism. Briefly, the DNA is extracted from whole or ground seed by incubation in the Extraction Solution at 56°C for 10 minutes, then put it in 95°C for 5 minutes. After an equal volume of the Dilution Solution is added to the extract to neutralize inhibitory substances, the extract is ready for PCR. An aliquot of the diluted extract is then combined with the 2 x TaqMasterMix and user provided PCR primers to amplify target DNA.  2 x Taq MasterMix is a 2 x Reaction Mix containing buffer, salts, dNTPs. and Taq DNA Polymerase. It is optimized specifically for use with the extraction reagents.