C4结合蛋白β(C4BPβ)重组蛋白

YB761Hu012
C4 Binding Binding Binding Binding Protein Protein Protein Protein Beta (C4BPb) (C4BPb) (C4BPb) (C4BPb)
Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human)
Instruction Instruction Instruction Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES
4th Edition (Revised in August, 2012)
 C4结合蛋白β(C4BPβ)重组蛋白Protein Protein Protein Protein Names: C4 Binding Protein Beta
Synonyms: Synonyms: Synonyms: Synonyms: C4BPb
Size: 100 µg
Source: Source: Source: Source: Escherichia coli-derived
Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Secreted.
[ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]
Residues Residues Residues Residues: Ser18~Leu252 (Accession # P20851), with a N-terminal his-tag.
Grade & Purity: Purity: Purity: Purity: >95%, 27.9 kDa as determined by SDS-PAGE reducing conditions.
Formulation: Formulation: Formulation: Formulation: Supplied as liquid form in Phosphate buffered saline(PBS),  C4结合蛋白β(C4BPβ)重组蛋白pH 7.4.

Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method).
Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 27.9 kDa
Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 5.3
[ PREPARATION PREPARATION PREPARATION PREPARATION ]
Reconstitute in PBS, PH7.2-PH7.4.
[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months .
Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was
determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious
degradation and precipitation were observed. (Referring from China Biological Products Standard, which was
calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under
appropriate storage condition.
[ BACKGROUND BACKGROUND BACKGROUND BACKGROUND]
The target protein is fused with a N-terminal his-tag. Their sequence is listed below.
MGHHHHHHSGSEF-SDA EHCPELPPVD NSIFVAKEVE GQILGTYVCI KGYHLVGKKT LFCNASKEWD  C4结合蛋白β(C4BPβ)重组蛋白NTTTECRLGH

CPDPVLVNGE FSSSGPVNVS DKITFMCNDH YILKGSNRSQ CLEDHTWAPP FPICKSRDCD PPGNPVHGYF EGNNFTLGST
ISYYCEDRYY LVGVQEQQCV DGEWSSALPV CKLIQEAPKP ECEKALLAFQ ESKNLCEAME NFMQQLKESG MTMEELKYSL
ELKKAELKAK LL
[ REFERENCES REFERENCES REFERENCES REFERENCES ]
1. Hillarp A., et al. (1990) Proc. Natl. Acad. Sci. U.S.A. 87:1183-1187.
2. Hillarp A., et al. (1993) J. Biol. Chem. 268:15017-15023.
3. Gregory S G., et al. (2006) Nature 441:315-321.
4. Mural R J., et al. (2005) Submitted to the EMBL/GenBank/DDBJ databases.