Product Description

Epithelial Cell Growth Supplement-2 (EpiCGS-2) is a medium supplement designed for the optimal growth of human normal esophageal epithelial cells in vitro. It is a sterile, concentrated (100X) solution which contains growth factors, hormones, and proteins necessary for the culture of normal human epithelial cells. The supplement is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced growth environment that maximally promotes the growth of normal human epithelial cells in vitro. The supplement is designed as an additive for epithelial cell medium-2 (EpiCM-2, Cat. No. 4111) and should be used in conjunction with that medium.

Components

EpiCGS-2 is packaged in the quantity of supplement suited for a 500 ml bottle of epithelial cell medium-2. When a 500 ml bottle of epithelial cell medium-2 is supplemented with EpiCGS-2, the final concentrations of the supplement components per milliliter will be BSA 5 μg, transferrin 5 μg, BPE 50 μg, insulin 2.5 mg, FGF-2 5 ng, EGF 1ng, Epinephrine 500 ng, hydrocortisone 0.5 mg, Prostaglandin E2 10-8 M and T3 30 nM. Note: Due to BPE in the growth supplement, formation of lipoproteins can cause precipitates to be present; the color may vary with different lots.

Product Use

EpiCGS-2 is for research use only.  It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Store the EpiCGS-2 at -20oC before adding to epithelial cell medium.

Shipping

Dry ice.

Prepare for use

Thaw EpiCGS-2 at 37oC. Gently tilt the EpiCGS-2 tube several times during thawing to help the contents dissolve. Make sure the contents of the supplement are completely dissolved into solution before adding to the medium. Rinse the bottle and tubes with 70% ethanol, and then wipe to remove excess. Remove the cap, being careful not to touch the interior threads with fingers. Add EpiCGS-2 and other components (FBS and P/S solution) into basal medium in a sterile field, mix well and then the reconstituted medium is ready for use. Since several components of epithelial cell medium are light-labile, it is recommended that the medium not be exposed to light for lengthy periods of time. If the medium is warmed prior to use, do not exceed 37oC. When stored in the dark at 4oC, the reconstituted medium is stable for one month.