CCNE1 (phospho T395) Peptide
产品名称： CCNE1 (phospho T395) Peptide
英文名称： CCNE1 (phospho T395) Peptide
产品编号： P1307
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
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- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of CCNE1.
- Immunogen:
- Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T395 of human CCNE1.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
Dot Blot (1:500)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- G1 arrest by p16INK4A uncouples growth from cell cycle progression in leukemia cells with deregulated cyclin E and c-Myc expression.
Ausserlechner MJ, Obexer P, Geley S, Kofler R.Leukemia. 2005 Jun;19(6):1051-7.
- 2.
- The tumor-specific hyperactive forms of cyclin E are resistant to inhibition by p21 and p27.
Wingate H, Zhang N, McGarhen MJ, Bedrosian I, Harper JW, Keyomarsi K.J Biol Chem. 2005 Apr 15;280(15):15148-57. Epub 2005 Feb 10.
- 3.
- The structure of cyclin E1/CDK2: implications for CDK2 activation and CDK2-independent roles.
Honda R, Lowe ED, Dubinina E, Skamnaki V, Cook A, Brown NR, Johnson LN.EMBO J. 2005 Feb 9;24(3):452-63. Epub 2005 Jan 20.
- Applications
- Western Blot (Cell lysate)
- The CCNE1 (phospho T395) polyclonal antibody (Cat # PAB0447) is used in Western blot to detect Phospho-CCNE1-T395 in HeLa cell lysate
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with CCNE1 (phospho T395) polyclonal antibody (Cat # PAB0447) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Entrez GeneID:
- 898
- Protein Accession#:
- NP_001229;P24864
- Gene Name:
- CCNE1
- Gene Alias:
- CCNE
- Gene Description:
- cyclin E1
- Omim ID:
- 123837
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available. [provided by RefSeq
- Other Designations:
- cyclin Es,cyclin Et
- Interactome
