TP53 polyclonal antibody-抗体-抗体-生物在线

产品名称： TP53 polyclonal antibody

英文名称： TP53 polyclonal antibody

产品编号： PAB4847

产品价格： null

产品产地：台湾

品牌商标： Abnova

更新时间： null

使用范围：

亚诺法生技股份有限公司（Abnova)

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Immunogen:
A synthetic peptide (conjugated with KLH) corresponding to residues surrounding S15 of human TP53.

Storage Instruction:
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

Recommend Usage:
ELISA (1:1000)
Western Blot (1:50-100)
The optimal working dilution should be determined by the end user.

Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

1.
Both BC-box motifs of adenovirus protein E4orf6 are required to efficiently assemble an E3 ligase complex that degrades p53.
Blanchette P, Cheng CY, Yan Q, Ketner G, Ornelles DA, Dobner T, Conaway RC, Conaway JW, Branton PE.Mol Cell Biol. 2004 Nov;24(21):9619-29.

2.
Identification of SCN3B as a novel p53-inducible proapoptotic gene.
Adachi K, Toyota M, Sasaki Y, Yamashita T, Ishida S, Ohe-Toyota M, Maruyama R, Hinoda Y, Saito T, Imai K, Kudo R, Tokino T.Oncogene. 2004 Oct 14;23(47):7791-8.

3.
Repression of hsp90beta gene by p53 in UV irradiation-induced apoptosis of Jurkat cells.
Zhang Y, Wang JS, Chen LL, Zhang Y, Cheng XK, Heng FY, Wu NH, Shen YF.J Biol Chem. 2004 Oct 8;279(41):42545-51. Epub 2004 Jul 28.

Western Blot (Transfected lysate)
Western blot analysis of TP53 (arrow) using rabbit TP53 polyclonal antibody (Cat # PAB4847). 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TP53 gene (Lane 2) (Origene Technologies).

Gene Summary:
This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity. [provided by RefSeq

Other Designations:
p53 antigen,p53 transformation suppressor,p53 tumor suppressor,phosphoprotein p53,transformation-related protein 53