CPSF4,剪切和多聚腺苷酸化特异性因子4抗体

产品编号HZ-2253R
英文名称CPSF4
中文名称剪切和多聚腺苷酸化特异性因子4抗体
别 名Cleavage and polyadenylation specificity factor 30 kDa subunit; CPSF 30 kDa subunit; CPSF30; CPSF4; NAR; NEB1; No arches homolog; NS1 effector domain binding protein 1; CPSF4_RAT.
说 明 书0.1ml 0.2ml
研究领域免疫学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,
CPSF4,剪切和多聚腺苷酸化特异性因子4抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量27kDa
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human CPSF4/CPSF30
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
CPSF4,剪切和多聚腺苷酸化特异性因子4抗体PubMedPubMed
产品介绍background:
Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs. Multiple alternatively spliced transcript variants that encode different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Function:
Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. CPSF4 binds RNA polymers with a preference for poly(U).

Subunit:
Component of the cleavage and polyadenylation specificity factor (CPSF) complex, composed of CPSF1, CPSF2, CPSF3, CPSF4 and FIP1L1. Interacts with FIP1L1.

Subcellular Location:
Nucleus.

CPSF4,剪切和多聚腺苷酸化特异性因子4抗体Similarity:
Belongs to the CPSF4/YTH1 family.
Contains 5 C3H1-type zinc fingers.
Contains 1 CCHC-type zinc finger.

Gene ID:
10898

Database links:
Entrez Gene: 10898 Human
Entrez Gene: 54188 Mouse
Entrez Gene: 304277 Rat
Omim: 603052 Human
SwissProt: O95639 Human
SwissProt: Q8BQZ5 Mouse
SwissProt: Q5FVR7 Rat
Unigene: 489287 Human
Unigene: 196884 Mouse
Unigene: 104788 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.