肽基精氨酸脱亚氨酶Ⅱ(PADI2)重组蛋白

YB707Hu014
Recombinant Peptidyl Arginine Deimina Type II (PADI2)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
 肽基精氨酸脱亚氨酶Ⅱ(PADI2)重组蛋白PROPERTIES ]
Residues: Met1~Thr300
Tags: Two N-terminal Tags, His-tag and GST-tag
Accession: Q9Y2J8
Host: E. coli
Subcellular Location: Cytoplasm.
Purity: >90%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
 肽基精氨酸脱亚氨酶Ⅱ(PADI2)重组蛋白pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 4.9
Predicted Molecular Mass: 63.9kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
 肽基精氨酸脱亚氨酶Ⅱ(PADI2)重组蛋白protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
MLRERTVRLQ YGSRVEAVYV LGTYLWTDVY SAAPAGAQTF SLKHSEHVWV EVVRDGEAEE
VATNGKQRWL LSPSTTLRVT MSQASTEASS DKVTVNYYDE EGSIPIDQAG LFLTAIEISL
DV DA DRD GVV EK NNP KKASW TWGPEG QGAI LLV NCD RETP WLPKE DCR DE
KVYSKEDLKD MSQMILRTKG PDRLPAGYEI VLYISMSDSD KVGVFYVENP FFGQRYIHIL
GRRKLYHVVK YTGGSAELLF FVEGLCFPDE GFSGLVSIHV SLLEYMAQDI PLTPIFTDTV
IFRIAPWIMT