NEDD9 monoclonal antibody, clone 14A11
产品名称： NEDD9 monoclonal antibody, clone 14A11
英文名称： NEDD9 monoclonal antibody, clone 14A11
产品编号： MAB2091
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
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- Specification
- Product Description:
- Mouse monoclonal antibody raised against partial recombinant NEDD9.
- Immunogen:
- Recombinant GST fusion protein corresponding to amino acids 82-398 of human NEDD9.
- Host:
- Mouse
- Reactivity:
- Human
- Specificity:
- This Protein A purified antibody is directed against human HEF1 protein. Reactivity against multiple isoforms is expected. Specificity was determined by partial epitope mapping.
- Form:
- Liquid
- Isotype:
- IgG2b, kappa
- Storage Buffer:
- In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
- Recommend Usage:
- Western Blot (1:500)
Immunofluorescence (1:100)
Immunoprecipitation (1:100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Crk-associated substrate (Cas) family member, NEDD9, is regulated in human neuroblastoma cells and in the embryonic hindbrain by all-trans retinoic acid.
Merrill RA, See AW, Wertheim ML, Clagett-Dame M.Dev Dyn. 2004 Nov;231(3):564-75.
- 2.
- Regulation of HEF1 expression and phosphorylation by TGF-beta 1 and cell adhesion.
Zheng M, McKeown-Longo PJ.J Biol Chem. 2002 Oct 18;277(42):39599-608. Epub 2002 Aug 19.
- 3.
- Human enhancer of filamentation 1, a novel p130cas-like docking protein, associates with focal adhesion kinase and induces pseudohyphal growth in Saccharomyces cerevisiae.
Law SF, Estojak J, Wang B, Mysliwiec T, Kruh G, Golemis EA.Mol Cell Biol. 1996 Jul;16(7):3327-37.
- Applications
- Western Blot (Cell lysate)
- Western blotting using NEDD9 monoclonal antibody, clone 14A11 (Cat # MAB2091) shows detection of NEDD9 present in MCF-7 cells induced to express NEDD9 by tetracycline removal (right lane).
See Pugacheva et al for details.
- Immunofluorescence
- Immunofluorescence microscopyusing NEDD9 monoclonal antibody, clone 14A11 (Cat # MAB2091) shows detectionof NEDD9 localized at the centrosome (bright dots) and focal adhesion sites.
The antibody was used at a 1:100 dilution.
Personal Communication. Elena Pugacheva, FoxChase Cancer Center, Philadelphia, PA.
- Entrez GeneID:
- 4739
- Protein Accession#:
- NP_006394;Q14511
- Gene Name:
- NEDD9
- Gene Alias:
- CAS-L,CAS2,CASL,CASS2,HEF1,dJ49G10.2,dJ761I2.1
- Gene Description:
- neural precursor cell expressed, developmentally down-regulated 9
- Omim ID:
- 602265
- Gene Ontology:
- Hyperlink
- Other Designations:
- Cas scaffolding protein family member 2,Crk-associated substrate related,OTTHUMP00000016030,cas-like docking,dJ49G10.2 (Enhancer of Filamentation 1 (HEF1)),dJ761I2.1 (enhancer of filamentation (HEF1)),enhancer of filamentation 1