Cdc42-GTP 小鼠单抗/Anti-Cdc42-GTP Monoclonal Antibody
产品名称： Cdc42-GTP 小鼠单抗/Anti-Cdc42-GTP Monoclonal Antibody
英文名称： Anti-Cdc42-GTP Monoclonal Antibody
产品编号： 26905
产品价格： null
产品产地： 美国
品牌商标： Neweast Biosciences
更新时间： null
使用范围： IP, IHC, IF
- 联系人 :
- 地址 : 武汉东湖新技术开发区高新大道666号武汉国家生物产业基地项目（生物创新园）B.C.D区研发楼B3-3栋3楼
- 邮编 :
- 所在区域 : 湖北
- 电话 : 181****2833 点击查看
- 传真 : 点击查看
- 邮箱 : nebio0923@163.com
| 货号: 26905 |
| 规格: 30 μL |
| 基因符号: CDC42 |
| 描述: Anti-Cdc42-GTP Monoclonal Antibody |
| 背景介绍: Small GTPases are a super-family of cellular signaling regulators. Cdc42 belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the activity of Cdc42, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). |
| 免疫原: Recombinant full length protein of active Cdc42 |
| 经过测试的应用: IP, IHC |
| 推荐稀释比: IP: 1 μg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
| 浓度: 1 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG1 |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Anti-Cdc42-GTP monoclonal antibody recognizes active Cdc42 from vertebrates. |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
IP-蛋白质印迹: IP/WB analysis of active Cdc42 protein. Purified recombinant Cdc42 proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated with anti-Cdc42-GTP mouse monoclonal antibody (Cat. # 26905). After SDS/PAGE, the membrane filter was probed with anti-Cdc42 mouse monoclonal antibody (Cat. # 26008). |
免疫荧光: Immunofluorescent labeling of Cdc42-GTP in MS1 mouse endothelial cells. The MS1 cells cultured on glass coverslips (Fisher) in DMEM containing 5% FBS were fixed with 4% paraformaldehyde for 10 minutes at RT (room temperature) and permeabilized in PBSN (PBS+0.1%NP40) for 15 minutes at RT. The cells were stained with primary antibody (Cdc42-GTP) at 1: 25 dilution in Invitrogen’s CAS-BLOCK (00-8120) reagent at RT for 1 hour. After brief washing in PBSN (3x5min), a secondary Alexa555 conjugated goat anti mouse antibody (Invitrogen A21424) was applied in 1: 200 in CAS-BLOCK at RT for 1 hour. The coverslips were then washed in PBSN (3x5min) and mounted using vectorsheld’s hard set mounting medium (H-1500), and examined using a Zeiss inverted fluorescence microscope. |
