SPRR1a,角质蛋白α抗体

产品编号HZ-11162R
英文名称SPRR1a
中文名称角质蛋白α抗体
别 名19 kDa pancornulin; Cornifin A; Cornifin alpha; CornifinA; Small proline rich protein 1A; Small proline rich protein IA; spr; SPR IA; SPRK; SPRR 1a; SPRR1A protein; SPR1A_HUMAN.
说 明 书0.1ml 0.2ml
研究领域免疫学 神经生物学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog,
SPRR1a,角质蛋白α抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量10kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human SPRR1a/Cornifin A
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
SPRR1a,角质蛋白α抗体PubMedPubMed
产品介绍background:
The small proline rich protein (SPRR) gene family encodes a conserved group of cornified envelope (CE) proteins that are part of the human epidermal differentiation complex (EDC). The formation of the cornified envelope during the late stages of epidermal differentiation is essential for epidermal barrier function and protects the body against environmental attack and water loss. Additionally, the expression of SPRR proteins is linked to keratinocyte terminal differentiation. The SPRR gene family, namely comprises three subclasses of genes, SPRR1 (which contains two members), SPRR2 (which contains eight members) and SPRR3 (which contains one member). SPRR1 is found predominantly in follicular epidermis and oral mucosa, SPRR2 is expressed coherently in follicular and interfollicular epidermis and SPRR3 is absent in epidermis and strongly expressed in internal squamous.

Function:
The ability of neurons to regenerate an axon after injury is determined by both the surrounding environment and factors intrinsic to the damaged neuron. Neuronal SPRR1A is likely a significant contributor to successful nerve regeneration. The small proline-rich repeat protein 1A (SPRR1A) is not detectable in uninjured neurons but is induced by >60-fold after peripheral axonal damage being localized to injured neurons and axons. SPRR1A is one of a group of epithelial differentiation genes, including s100c and p21/waf, that are cocurrently induced in neurons by axotomy. Overexpressed SPRR1A colocalizes with F-actin in membrane ruffles and augments axonal outgrowth on a range of substrates. In axotomized sensory neurons, reduction of SPRR1A function restricts axonal outgrowth.

Subcellular Location:
Cytoplasmic

SPRR1a,角质蛋白α抗体Similarity:
Belongs to the cornifin (SPRR) family.

Database links:
Entrez Gene: 6698 Human
Omim: 182265 Human
SwissProt: P35321 Human
Unigene: 46320 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: mouse skin tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-SPRR1a Polyclonal Antibody, Unconjugated(HZ-11162R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human laryngo carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-SPRR1a Polyclonal Antibody, Unconjugated(HZ-11162R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining