肌球蛋白重链2(MYH2)重组蛋白

YB755Mu021
Recombinant Myosin Heavy Chain 2, Skeletal Muscle, Adult (MYH2)
Organism Species: Mus musculus (Mouse)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
 肌球蛋白重链2(MYH2)重组蛋白[ PROPERTIES ]
Residues: Leu1238~Ala1472 (Accession # I6L963),
with two N-terminal Tags, His-tag and GST-tag. Host: E. coli
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 5.8
Predicted Molecular Mass: 59.1kDa
 肌球蛋白重链2(MYH2)重组蛋白Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
 肌球蛋白重链2(MYH2)重组蛋白[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its
sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID
GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV
DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK
KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR
GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF -LAS NVETVSKAKG
NLEKMCRTLE DQVSELKSKE EEQQRLINDL TSQRGRLQTE SGEFSRQLDE KEALVSQLSR
GKQAFTQQIE ELKRQLEEEV KAKNALAHAL QSSRHDCDLL REQYEEEQES KAELQRALSK
ANSEVAQWRT KYETDAIQRT EELEEAKKKL AQRLQAAEEH VEAVNAKCAS LEKTKQRLQN
EVEDLMLDVE RTNAACAALD KKQRNFDKIL AEWKQKYEET HA