Phospho-NMDAR2A + 2B (Tyr1246 + Tyr1252),磷酸化谷氨酸受体2A+2B抗体

产品编号HZ-8567R
英文名称Phospho-NMDAR2A + 2B (Tyr1246 + Tyr1252)
中文名称磷酸化谷氨酸受体2A+2B抗体
别 名NMDAR2A + 2B (phospho Y1246 + Y1252); NR2B; NMDA2B (epsilon 2); GRIN 2B; GRIN2B; hNR 3; hNR3; MGC142178; MGC142180; N methyl D asparate receptor channel subunit epsilon 2; N METHYL D ASPARTATE RECEPTOR CHANNEL SUBUNIT EPSILON 2; N methyl D aspartate receptor subtype 2B; N methyl D aspartate receptor subunit 2B; N methyl D aspartate receptor subunit 3; NMDA NR2B; NMDA R2B; Nmdar2b; NMDE2; NME2; NR2B; NR3;N-Methyl-d-Asprtate receptor 2B; AW490526; Glutamate [NMDA] receptor subunit epsilon 2; Glutamate Receptor Ionotropic N Methyl D Aspartate 2B; Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B; Glutamate receptor ionotropic NMDA2B; Glutamate receptor subunit epsilon 2; Glutamate receptor, ionotropic; NMDE1_HUMAN/ NMDE2_HUMAN.
说 明 书0.1ml
产品类型磷酸化抗体
研究领域细胞生物 免疫学 神经生物学 细胞凋亡 细胞周期蛋白 细胞膜受体
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,
Phospho-NMDAR2A + 2B (Tyr1246 + Tyr1252),磷酸化谷氨酸受体2A+2B抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量163kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human NMDAR2A around the phosphorylation site of Tyr1246
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Phospho-NMDAR2A + 2B (Tyr1246 + Tyr1252),磷酸化谷氨酸受体2A+2B抗体PubMedPubMed
产品介绍background:
N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate-gated ion channels. These receptors have been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C) and NMDAR2D (GRIN2D). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Function:
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.

Subunit:
Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Found in a complex with GRIN1 and GRIN3B. Found in a complex with GRIN1, GRIN3A and PPP2CB. Interacts with PDZ domains of INADL and DLG4. Interacts with HIP1 and NETO1 (By similarity). Interacts with MAGI3. Interacts with DAPK1

Subcellular Location:
Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.

Similarity:
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain.

Gene ID:
2903
2904

Database links:
Entrez Gene: 2903 Human
Entrez Gene: 14811 Mouse
Entrez Gene: 24409 Rat
Omim: 138253 Human
SwissProt: Q12879 Human
SwissProt: P35436 Mouse
SwissProt: Q00959 Rat
Unigene: 411472 Human
Unigene: 2953 Mouse
Unigene: 9710 Rat

Phospho-NMDAR2A + 2B (Tyr1246 + Tyr1252),磷酸化谷氨酸受体2A+2B抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Phospho-NMDAR2A+2B(Tyr1246+Tyr1252) Polyclonal Antibody, Unconjugated (HZ-8567R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Phospho-NMDAR2A+2B(Tyr1246+Tyr1252) Polyclonal Antibody, Unconjugated (HZ-8567R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(HZ-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml, blue, C-0033) was used to stain the cell nuclei