Complement C4 / C4a,过敏毒素C4/补体C4抗体

产品编号HZ-11274R
英文名称Complement C4 / C4a
中文名称过敏毒素C4/补体C4抗体
别 名C4a anaphylatoxin; Complement C4-A alpha chain; complement C4-A proprotein; Acidic C4; Acidic complement C4; Basic C4; Basic complement C4; C4 Anaphylatoxin; C4 complement C4d region; complement C4 alpha chain; C4A; C4A13; C4A2; C4A3; C4A4; C4A6; C4A91; C4B; C4B1; C4B12; C4B2; C4B3; C4B5; C4F; C4S; CH; Chido form of C4; CO4; Complement C4 A; Complement C4 B; Complement C4B; Complement component 4A (Rodgers blood group); Complement component 4A; Complement component 4B (Childo blood group); Complement component 4B; Complement component C4B; CPAMD2; CPAMD3. RG; Rodgers Form Of C4.
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human,
Complement C4 / C4a,过敏毒素C4/补体C4抗体产品应用ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量8/84/190kDa
细胞定位分泌型蛋白
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Complement C4 C4a anaphylatoxin or complement C4 alpha chain
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Complement C4 / C4a,过敏毒素C4/补体C4抗体PubMedPubMed
产品介绍background:
This gene encodes the acidic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus and type I diabetes mellitus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011].

Function:
C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin.

Subunit:
This protein is synthesized as a single-chain precursor and, prior to secretion, is enzymatically cleaved to form a trimer of non-identical chains (alpha, beta and gamma).

Subcellular Location:
Secreted.

Tissue Specificity:

DISEASE:
Defects in C4A are the cause of complement component 4A deficiency (C4AD). A rare defect of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus with or without associated glomerulonephritis.

Similarity:
Contains 1 anaphylatoxin-like domain.
Contains 1 NTR domain.

Gene ID:
720

Complement C4 / C4a,过敏毒素C4/补体C4抗体Database links:
Entrez Gene: 100293534 Human
Entrez Gene: 100507685 Human
Entrez Gene: 720 Human
Entrez Gene: 721 Human
Omim: 120810 Human
SwissProt: P0C0L4 Human
SwissProt: P0C0L5 Human
Unigene: 534847 Human
Unigene: 720022 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: mouse intestine tissue;4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37鈩� for 20 min;  Incubation: Anti-Complement C4/C4 Polyclonal Antibody, Unconjugated(bs-11274R) 1:200, overnight at 4掳C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (HZ-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37掳C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei Tissue/cell: human lung cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti- Complement C4, Unconjugated(HZ-11274R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining