干扰素α11(IFNα11)重组蛋白

YB090Mu013
Recombinant Interferon Alpha 11 (IFNa11)
Organism Species: Mus musculus (Mouse)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
 干扰素α11(IFNα11)重组蛋白[ PROPERTIES ]
Residues: Cys24~Ala190
Tags: Two N-terminal Tags, His-tag and GST-tag
Accession: Q61716
Host: E. coli
Subcellular Location: Secreted.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
 干扰素α11(IFNα11)重组蛋白Formulation: Supplied as lyophilized form in 20mM Tris,
150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 8.9
Predicted Molecular Mass: 49.0kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
 干扰素α11(IFNα11)重组蛋白Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
CDLPHTY NLRNKRALKV LAQMRRLSPL SCLKDRQDFG FPLEKVDAQQ IKKAQAIPVL
RDLTQQILNL FTSKASSAAW NATLLDSFCN DLHQQLNDLQ GCLMQQVGVQ EPPLTQEDAL
LAVRIYFHSI TVFLREKKHS PCAWEVVRAE VWRALSSSVN LLARLSEEKA
[ REFERENCES ]
1. Coulombel C., et al. (1991) Gene 104:187-195. 2. van Pesch V., et al. (2004) J. Virol. 78:8219-8228. 3. Church D.M., et al. (2009) PLoS Biol. 7:E1000112-E1000112. 4. Gibbert K., et al. (2012) PLoS Pathog. 8:E1002868-E1002868.