重组人DNAM-1蛋白（Recombinant Human DNAM-1 Protein），GMP级

产品货号：GMP-TL718

产品描述（Description）

DNAX accessory molecule 1 (DNAM-1), a single-pass type I membrane protein, is also known as CD226 antigen and platelet and T cell activation antigen 1 (PTA1), which contains 2 Ig-like C2-type (immunoglobulin-like) domains. DNAM-1 is a ~65 kDa glycoprotein expressed on the surface of natural killer cells, platelets, monocytes and a subset of T cells. DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.

分子别名（Synonym）

DNAM1、CD226、PTA1

表达宿主（Expression host）

HEK293细胞

来源（Source）

Human

蛋白序列（Protein Sequence）

DNA序列编码人DNAM-1（NCBI Reference Sequence: NP_006557.2）表达带有His标签在C末端

纯度（Purity）

≥95%采用 SDS-PAGE 凝胶和高效液相色谱分析

内毒素（Endotoxin）

＜0.1EU/ug

分子量（Molecular Weight）

重组人DNAM-1蛋白 包含229个氨基酸，预测理论分子量为27.7 kD

生物活性（Biological Activity）

固定人 DNAM-1(His Tag)5ug/mL(100uL/孔) 能够结合 CD155(Fc Tag)的线性范围是 5-80ng/mL

稳定性（Stability）

冻干的样本的可以在4℃保存24个月，溶解后的液体可以于-20℃保存6-12个月，并且避免反复冻融

组成（Component）

含有6%甘露醇的无菌PBS（PH 7.4）的冻干粉

参考文献

1、DNAM-1 controls NK cell activation via an ITT-like motif Zhanguang Zhang, Ning Wu, Yan Lu, Dominique Davidson, Marco Colonna, André Veillette J Exp Med. 2015 Nov 16; 212(12): 2165–2182. doi: 10.1084/jem.20150792 

2、Expression of CD226 is associated to but not required for NK cell education Arnika K. Wagner, Nadir Kadri, Johanna Snäll, Petter Brodin, Susan Gilfillan, Marco Colonna, Günter Bernhardt, Petter Höglund, Klas Kärre, Benedict J. Chambers Nat Commun. 2017; 8: 15627. Published online 2017 May 31. doi: 10.1038/ncomms15627 

3.、CD8 T cell defect of TNF-α and IL-2 in DNAM-1 deficient mice delays clearance in vivo of a persistent virus infection Megan J. Welch, John R. Teijaro, Hanna A. Lewicki, Marco Colonna, Michael B.A. Oldstone Virology. Author manuscript; available in PMC 2013 Aug 1.Published in final edited form as: Virology. 2012 Aug 1; 429(2): 163–170. Published online 2012 May 12. doi: 10.1016/j.virol.2012.04.006

  注意：For research and manufacturing use.