phospho-ATG4A,磷酸化自噬相关蛋白4A抗体

产品编号bs-5207R
英文名称phospho-ATG4A(Ser100)
中文名称磷酸化自噬相关蛋白4A抗体
别 名AI627006; Apg4a; ATG4 autophagy related 4 homolog A (S. cerevisiae); ATG4A; ATG4A_HUMAN; Atg4al; AUT like 2 cysteine endopeptidase; AUT-like 2 cysteine endopeptidase; Autl2; Autophagin 2; Autophagin-2; Autophagy related 4A cysteine peptidase; Autophagy related cysteine endopeptidase 2; Autophagy related protein 4 homolog A; Autophagy-related cysteine endopeptidase 2; Autophagy-related protein 4 homolog A; AV169859; Cysteine protease ATG4A; hAPG4A; MGC107179.
规格价格0.1ml 
说 明 书0.1ml
产品类型磷酸化抗体
研究领域肿瘤 心血管 细胞生物 免疫学 信号转导 细胞凋亡 线粒体 细胞外基质 泛素
phospho-ATG4A,磷酸化自噬相关蛋白4A抗体抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human,
产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量45kDa
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human ATG4A around the phosphorylation site of Ser100
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
phospho-ATG4A,磷酸化自噬相关蛋白4A抗体PubMedPubMed
产品介绍background:
ATG4A is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.

Function:
Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP.

Subcellular Location:
Cytoplasm (Probable).

Tissue Specificity:
Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver.

Similarity:
Belongs to the peptidase C54 family.

Database links:
UniProtKB/Swiss-Prot: Q8WYN0.1

phospho-ATG4A,磷酸化自噬相关蛋白4A抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.