肌动蛋白β(ACTβ)重组蛋白

YB340Mi011
Recombinant Actin Beta (ACTb)
Organism Species: Homo sapiens (Human)
Mus musculus (Mouse)
Rattus norvegicus (Rat)
Cavia (Guinea pig)
Ovis aries; Ovine (Sheep)
Canis familiaris; Canine (Dog)
肌动蛋白β(ACTβ)重组蛋白Sus scrofa; Porcine (Pig)
Equus caballus; Equine (Horse)
Chicken (Gallus)
Bos taurus; Bovine (Cattle)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
12th Edition (Revised in Aug, 2016)
肌动蛋白β(ACTβ)重组蛋白[ PROPERTIES ]
Source: Prokaryotic expression. Host: E. coli
Residues: Met1~Phe375
Tags: N-terminal His-Tag
Subcellular Location: Cytoplasm, cytoskeleton. Purity: >92%
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200ug/mL
Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive
Labeling. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 5.6
Predicted Molecular Mass: 43.3kDa
Accurate Molecular Mass: 44kDa as determined by SDS-PAGE reducing conditions. Note: 100% cross-reactivity of ACTb was observed among human, mouse, rat, cavia, ovine, canine, porcine, equine, gallus, and bovine. [ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0
mg/mL. Do not vortex. [ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8
oC for one month. Aliquot and store at -80
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
肌动蛋白β(ACTβ)重组蛋白was determined by accelerated thermal degradation test, that is, incubate the
protein at 37
oC for 48h, and no obvious degradation and precipitation were
observed.The loss rate is less than 5% within the expiration date under
appropriate storage condition. [ SEQUENCE ]
[ IDENTIFICATION ]
Figure 1. Gene Sequencing (Extract)
Figure 2. SDS-PAGE