70kDaζ链关联蛋白激酶(ζAP70)重组蛋白

YB541Mu012
Zeta Chain Associated Associated Associated Associated Protein Protein Protein Protein Kinase 70kDa (zAP70) (zAP70) (zAP70) (zAP70)
Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human)
Instruction Instruction Instruction Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
 70kDaζ链关联蛋白激酶(ζAP70)重组蛋白7th Edition (Revised in May, 2013)
[ PROPERTIES PROPERTIES PROPERTIES PROPERTIES ]
Residues: Residues: Residues: Residues: Leu337~Leu600 (Accession # P43403),
with two N-terminal Tags, His-tag and T7-tag. Host: E. coli
Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Cytoplasm. Cell
membrane; Peripheral membrane protein.
Purity: Purity: Purity: Purity: >95%
Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
 70kDaζ链关联蛋白激酶(ζAP70)重组蛋白Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 8.6
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 34.2kDa
Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
 70kDaζ链关联蛋白激酶(ζAP70)重组蛋白[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its
sequence is listed below.
MGSSHHHHHH SSG LVPRGSH MASMTGGQQM GRGSEF -L LIA DIE LGCGNFG
SVRQGVYRMR KKQIDVAIKV LKQGTEKADT EEMMREAQIM HQLDNPYIVR LIGVCQAEAL
MLVMEMAGGG PLHKFLVGKR EEIPVSNVAE LLHQVSMGMK YLEEKNFVHR DLAARNVLLV
NRHYAKISDF GLSKALGADD SYYTARSAGK WPLKWYAPEC INFRKFSSRS DVWSYGVTMW
EALSYGQKPY KKMKGPEVMA FIEQGKRMEC PPECPPELYA LMSDCWIYKW EDRPDFLTVE
QRMRACYYSL