The Solar.® Cycle-Pure Kit is designed for the rapid purification of single or double stranded DNA from PCR or other enzymatic reactions. The purification procedure completely removes primers, nucleotides enzymes, salts, and other impurities from DNA samples. The DNA sample is simply mixed with CPP buffer and spun through the HiBind® DNA column. The DNA bound to the HiBind® Matrix is washed and the cleaned concentrated DNA is eluted with deionized water or EB Buffer. This convenient spin-column format eliminates the need for expensive resins or toxic organic compounds such as phenol and chloroform, thereby making it possible to process multiple samples in parallel. Purified DNA can be used in T-A ligations, sequencing, restriction enzyme digestion, and various other labeling reactions.

Analysis of PCR products before (b) and after (a) purfication with the Solar.® Cycle-Pure Kit Samples

were analyzed on 1.5% TBE agarose gel.

M1: 100 bp DNA Marker

M2: High throughput DNA Marker I