DICER1抗体
Applications
| Tested Applications: |
ELISA, WB, IHC, IF, IP |
| Cited applications |
WB |
| Specificity Reaction: |
Human,Mouse,Rat; Other species are not tested.Please decide the specificity by homology |
| Cited species |
Human, mouse |
| Positive WB detected in |
K-562 cells, Jurkat cells |
| Positive IP detected in |
Jurkat cells |
| Positive IHC detected in |
Human testis tissue, human lung cancer tissue |
| Positive IF detected in |
HepG2 cells |
| Recommended dilution |
WB : 1:200-1:1000
IP : 1:200-1:1000
IHC : 1:20-1:200
IF : 1:10-1:100 |
DICER1抗体Application key: WB=Western Blotting ,IHC=Immunohistochemistry, IF=Immunofluorescence
Images
Prev12Next
K-562 cells were subjected to SDS PAGE followed by western blot with 20567-1-AP(DICER1 antibody) at dilution of 1:200
IP Result of anti-DICER1 (IP:20567-1-AP, 5ug; Detection:20567-1-AP 1:300) with Jurkat cells lysate 2000ug.
Immunohistochemistry of paraffin-embedded human testis tissue slide using 20567-1-AP( DICER1 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using 20567-1-AP( DICER1 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
Product Information DICER1抗体
| Source: |
Rabbit |
Purify: |
Antigen affinity purification |
| IsoType: |
IgG |
Storage: |
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. -20oC, Avoid freeze / thaw cycles. |
Immunogen Information
| Immunogen: |
Peptide |
Full Name: |
Dicer 1, ribonuclease type III |
Caculated Molecular
Weight: |
219 kDa |
Observed Molecular
Weight: |
220-250kd,90kd |
| GenbankNo |
NM_030621 |
GeneID: |
23405 |
| Symbol |
DICER1 |
Synonyms |
DCR1, Dicer, dicer 1, ribonuclease type III, DICER1, Endoribonuclease Dicer, Helicase MOI, Helicase with RNase motif, HERNA, KIAA0928 |
Background
|
DICER1, also named as DICER, HERNA and KIAA0928, belongs to the helicase family and Dicer subfamily. It is required for formation of the RNA induced silencing complex (RISC). DICER1 is a component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. DICER1 cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs. The antibody is specific to DICER1. DICER1抗体
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