重组人PD-L2蛋白（Recombinant Human PD-L2 Protein），GMP级

产品货号：GMP-TL728

产品描述（Description）

Programmed cell death 1 ligand 2 (PD-L2 or PDCD1 ligand 2) is also known as Butyrophilin B7-DC, CD antigen CD273, which belongs to the immunoglobulin superfamily or BTN/MOG family. The expression of PD-L2 is up-regulated by IFNG/IFN-gamma stimulation in monocytes and induced on dendritic cells grown from peripheral blood mononuclear cells with CSF2 and IL-4. PD-L2 Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. PD-L2 interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.

分子别名（Synonym）

PDL2、Butyrophilin B7-DC、CD273、PDCD1 ligand 2、PDCD1L2、PDCD1LG2

表达宿主（Expression host）

HEK293细胞

来源（Source）

Human

纯度（Purity）

≥95%采用SDS-PAGE凝胶和高效液相色谱分析

内毒素（Endotoxin）

＜0.1EU/ug

蛋白序列（Protein Sequence）

DNA序列编码人PD-L2（NCBI Reference Sequence:NP_079515.2）表达带有His标签在C末端

分子量（Molecular Weight） 

重组人PD-L2蛋白 包含201个氨基酸，预测理论分子量为24kD

生物活性（Biological Activity）

固定人(His 标签)10ug/mL( 100 ul/孔)能够结合人 PD-1(Fc 标签)的线性范围是 0.01-1ug/mL

稳定性（Stability）

冻干的样本的可以在4℃保存24个月，溶解后的液体可以于-20℃保存6-12个月，并且避免反复冻融

组成（Component）

含有6%甘露醇的无菌PBS（PH 7.4）的冻干粉

参考文献

1、PD-L2 regulates B-1 cell antibody production against phosphorylcholine through an IL-5–dependent mechanism Jerome T. McKay, Marcela A. Haro, Christina A. Daly, Rama D. Yammani, Bing Pang, W. Edward Swords, Karen M. Haas J Immunol. Author manuscript; available in PMC 2018 Sep 15. Published in final edited form as: J Immunol. 2017 Sep 15; 199(6): 2020–2029. Published online 2017 Aug 2. doi: 10.4049/jimmunol.1700555 

2、PD-L2 expression in colorectal cancer: Independent prognostic effect and targetability by deglycosylation Huanbin Wang, Han Yao, Chushu Li, Lunxi Liang, Yao Zhang, Hubing Shi, Chongzhi Zhou, Yingxuan Chen, Jing-Yuan Fang, Jie Xu Oncoimmunology. 2017; 6(7): e1327494. Published online 2017 May 16. doi: 10.1080/2162402X.2017.1327494

   注意：For research and manufacturing use.