Casp2 polyclonal antibody
产品名称： Casp2 polyclonal antibody
英文名称： Casp2 polyclonal antibody
产品编号： PAB10086
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of Casp2.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of rat Casp2.
- Host:
- Rabbit
- Reactivity:
- Mouse, Rat
- Form:
- Liquid
- Storage Buffer:
- In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- ELISA (1:1500-1:6000)
Western Blot (1:750)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Defects in regulation of apoptosis in caspase-2-deficient mice.
Bergeron L, Perez GI, Macdonald G, Shi L, Sun Y, Jurisicova A, Varmuza S, Latham KE, Flaws JA, Salter JC, Hara H, Moskowitz MA, Li E, Greenberg A, Tilly JL, Yuan J.Genes Dev. 1998 May 1;12(9):1304-14.
- 2.
- Activation of caspase-2 in apoptosis.
Li H, Bergeron L, Cryns V, Pasternack MS, Zhu H, Shi L, Greenberg A, Yuan J.J Biol Chem. 1997 Aug 22;272(34):21010-7.
- 3.
- Caspases: the executioners of apoptosis.
Cohen GM.Biochem J. 1997 Aug 15;326 ( Pt 1):1-16.
- Applications
- Western Blot (Transfected lysate)
- Immunoblotting of Casp2 polyclonal antibody (Cat # PAB10086) was used at a 1:750 dilution to detect rat Casp2 in transfected human 293 cell lysates by Western blot.
Asterisk indicates a 48 kDa Casp2 protein clearly detected in the rat Casp2 transfected lysate.
No reaction is observed against human Casp2.
Control (lane 1) and transfected (lane 2) lysates were loaded on a 4-20% gel for SDS-PAGE.
After primary antibody incubation and washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development using Amersham's substrate system.