SIRT1 抗体Applications
| In-house applications |
ELISA, IP, IHC, WB, IF Application not tested? Please see our Guarantee. |
| Published applications |
IF, IHC, IP, WB |
Publications |
18 citations –view here |
| Species specificity |
Human,Mouse,Rat; other species not tested. |
| Published Species |
Human, mouse, rat |
|
Images
Prev12Next
 HeLa cells were subjected to SDS PAGE followed by western blot with 13161-1-AP( SIRT1 Antibody) at dilution of 1:800 IP Result of anti-SIRT1 (IP:13161-1-AP, 4ug; Detection:13161-1-AP 1:500) with mouse testis tissue lysate 8000ug. Immunohistochemistry of paraffin-embedded mouse testis tissue slide using 13161-1-AP( SIRT1 Antibody) at dilution of 1:50 (under 40x lens) Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 13161-1-AP( SIRT1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
|
| Positive WB detected in |
HeLa cells, DU 145 cells, HEK-293 cells, Jurkat cells, K-562 cells, MCF7 cells, mouse testis tissue, PC-3 cells |
| Positive IP detected in |
Mouse testis tissue |
| Positive IHC detected in |
Mouse testis tissue, human colon cancer tissue, human lung cancer tissue |
| Positive IF detected in |
HeLa cells |
| Recommended dilution |
WB : 1:200-1:2000
IP : 1:200-1:2000
IHC : 1:50-1:200
IF : 1:50-1:500 |
SIRT1 抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
| Source |
Rabbit |
Purification method |
Antigen affinity purification |
| Isotype |
IgG |
Storage |
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information SIRT1 抗体
| Immunogen |
SIRT1 fusion protein ag3808 |
Full name |
Sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) |
Calculated molecular
weight |
747aa,82 kDa |
Observed molecular
weight |
110-130 kDa,80-85 kDa |
GenBank accession
number |
BC012499 |
Gene ID (NCBI) |
23411 |
| Gene symbol |
SIRT1 |
Synonyms |
HSIR2, hSIRT1, SIR2 like protein 1, SIR2L1, SIRT1 |
Background
|
SIRT1, also named as SIR2L1, contains a deacetylase sirtuin-type domain and belongs to the sirtuin family. The post-translation modified SIRT1 is a 110 kDa protein, which contains one deacetylase sirtuin-type domain. The 75 kda SirT1 fragment was detected to lack the carboxy-terminus (PMID:21305533). SIRT1 may be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane. It can shuttles between nucleus and cytoplasm. SIRT1 regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. SIRT1 in particular initiates several signaling events relevant to cardioprotection, including: activation of endothelial nitric oxide synthase, insulin receptor signaling, and autophagy. In addition SIRT1 activation elicits resistance to oxidative stress via regulation of transcription factors and co-activators such as FOXO, Hif-2a, and NF-kB. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SIRT1. SIRT1 抗体
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XY560Hu22 表皮生长因子(EGF)单克隆抗体 Monoclonal Antibody to Epiderkl Growth Factor (EGF) Homo sapiens (Hukl)
