The Plant Direct PCR Kit contains all of the reagents required to rapidly extract and amplify genomic DNA from plant leaves. Briefly, the DNA is extracted from a piece of leaf tissue, a 0.5 to 0.7 cm disk cut with a standard paper punch, by incubation in the Extraction Solution at 55°C for 10 minutes, then put it in 95°C for 5 minutes. After an equal volume of the PT3 Buffer is added to the extract to neutralize inhibitory substances, the extract is ready for PCR. An aliquot of the diluted extract is then combined with the 2 x  TaqMasterMix and user provided PCR primers to amplify target DNA. 2 x Taq Master Mix is a 2 x Reaction Mix containing buffer, salts, dNTPs, and Taq DNA Polymerase. It is optimized specifically for use with the extraction reagents.